Glial glutamate transporters and maturation of the mouse somatosensory cortex

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Serval ID
serval:BIB_49FE2E9767AB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glial glutamate transporters and maturation of the mouse somatosensory cortex
Journal
Cerebral Cortex
Author(s)
Voutsinos-Porche  B., Knott  G., Tanaka  K., Quairiaux  C., Welker  E., Bonvento  G.
ISSN
1047-3211 (Print)
Publication state
Published
Issued date
10/2003
Volume
13
Number
10
Pages
1110-21
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
In the adult nervous system, glutamatergic neurotransmission is tightly controlled by neuron-glia interactions through glial glutamate reuptake by the specific transporters GLT-1 and GLAST. Here, we have explored the role of these transporters in the structural and functional maturation of the somatosensory cortex of the mouse. We provide evidence that GLT-1 and GLAST are early and selectively expressed in barrels from P5 to P10. Confocal and electron microscopy confirm that the expression is restricted to the astroglial membrane. By P12, and despite an increased global expression as observed by immunoblotting, the barrel pattern of GLAST and GLT-1 staining is no longer evident. In P10 GLT-1 -/- and GLAST -/- mice, the cytoarchitectural segregation of the barrels is preserved. However, at P9-10, the functional response to whisker stimulation, measured by deoxyglucose uptake, is markedly decreased in GLT-1 -/- and GLAST -/- mice. The role of GLAST is transient since the metabolic response is already restored at P11-12 in GLAST -/- mice and remains unchanged in adulthood. However, deletion of GLT-1 seems to impair the functional metabolic response until adulthood. Our data suggest that astrocyte-neuron interactions via the glial glutamate transporters are involved in the functional maturation of the whisker representation in the somatosensory cortex.
Keywords
Amino Acid Transport System X-AG/*biosynthesis/genetics/ultrastructure Animals Animals, Newborn Excitatory Amino Acid Transporter 2/*biosynthesis/genetics/ultrastructure Female Mice Mice, Inbred C57BL Mice, Knockout Neuroglia/chemistry/*metabolism/ultrastructure Pregnancy Somatosensory Cortex/chemistry/*growth & development/*metabolism/ultrastructure
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:40
Last modification date
25/09/2019 6:09
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