Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus

Details

Serval ID
serval:BIB_49DACDE1668C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Influence of a functional sigB operon on the global regulators sar and agr in Staphylococcus aureus
Journal
Journal of Bacteriology
Author(s)
Bischoff  M., Entenza  J. M., Giachino  P.
ISSN
0021-9193 (Print)
Publication state
Published
Issued date
09/2001
Volume
183
Number
17
Pages
5171-9
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep
Abstract
The growth phase-dependent activity profile of the alternate transcription factor sigma(B) and its effects on the expression of sar and agr were examined in three different Staphylococcus aureus strains by Northern blot analyses and by the use of reporter gene fusion experiments. Significant sigma(B) activity was detectable only in the clinical isolates MSSA1112 and Newman, carrying the wild-type rsbU allele, but not in the NCTC8325 derivative BB255, which is defective in rsbU. sigma(B) activity peaked in the late exponential phase and diminished towards the stationary phase when bacteria were grown in Luria-Bertani medium. Transcriptional analysis and a sarP1-sarP2-sarP3 (sarP1-P2-P3)-driven firefly luciferase (luc+) reporter gene fusion demonstrated a strong sigma(B) activity- and growth phase-dependent increase in sar expression that was totally absent in either rsbU or Delta rsbUVWsigB mutants. In contrast, expression of the agr locus, as measured by RNAIII levels and by an hldp::luc+ fusion, was found to be higher in the absence of sigma(B) activity, such as in rsbU or Delta rsbUVWsigB mutants, than in wild-type strains. Overexpression of sigma(B) in BB255 derivatives resulted in a clear increase in sarP1-P2-P3::luc+ expression as well as a strong decrease in hldp::luc+ expression. The data presented here suggest that sigma(B) increases sar expression while simultaneously reducing the RNAIII level in a growth phase-dependent manner.
Keywords
Alleles Bacterial Proteins/*genetics Fibronectins/metabolism Genes, Reporter Operon *Phosphoric Monoester Hydrolases RNA, Antisense/metabolism RNA, Bacterial/metabolism Sigma Factor/*genetics Staphylococcus aureus/*genetics *Trans-Activators Transcription Factors/*genetics Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:45
Last modification date
20/08/2019 13:57
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