Effect of length and location of heterologous sequences on RecA-mediated strand exchange.

Details

Serval ID
serval:BIB_48F5E0BE5650
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of length and location of heterologous sequences on RecA-mediated strand exchange.
Journal
Journal of Biological Chemistry
Author(s)
Morel P., Stasiak A., Ehrlich S.D., Cassuto E.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
08/1994
Volume
269
Number
31
Pages
19830-19835
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We systematically investigated the effect of heterology on RecA-mediated strand exchange between double-stranded linear and single-stranded circular DNA. Strand exchange took place through heterologies of up to 150-200 base pairs when the insertion was at the proximal (initiating) end of the duplex DNA but was completely blocked by an insert of only 22 base pairs placed at the distal end of the duplex. In the case of medial heterology created by insertion either in the duplex or the single-stranded DNA, the ability of RecA to exchange strands decreased as the heterology was shifted toward the distal end of the duplex. These results suggest that two different strand exchange mechanisms operate in the proximal and distal portions of the duplex substrate.
Keywords
Bacteriophage M13, DNA/metabolism, DNA, Circular/metabolism, DNA, Viral/metabolism, DNA-Binding Proteins/metabolism, Nucleic Acid Conformation, Rec A Recombinases/genetics, Rec A Recombinases/metabolism
Pubmed
Web of science
Create date
24/01/2008 10:36
Last modification date
20/08/2019 13:56
Usage data