High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry.

Details

Serval ID
serval:BIB_48CFDB64F60C
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
High-Throughput, Fast, and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry.
Journal
Methods in molecular biology
Author(s)
Marino F., Chong C., Michaux J., Bassani-Sternberg M.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
1913
Pages
67-79
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Comprehensive knowledge of the HLA class I and class II peptides presented to T cells is crucial for designing innovative therapeutics against cancer and other diseases. So far, methodologies for recovery of HLA class I and II peptides for subsequent mass spectrometry-based analysis have been a major limitation. In this chapter we describe a detailed protocol for a high-throughput, reproducible, and sensitive immunoaffinity-purification of HLA-I and HLA-II peptides from up to 96 samples in a plate format, suitable for tissue samples and cell lines. Our methodology reduces sample handling, has a competitive peptide yield, and can be completed within 5 h. This simplified pipeline is applicable for basic and clinical applications.
Keywords
Cell Line, Chromatography, High Pressure Liquid/instrumentation, Chromatography, High Pressure Liquid/methods, High-Throughput Screening Assays/instrumentation, High-Throughput Screening Assays/methods, Histocompatibility Antigens Class I/immunology, Histocompatibility Antigens Class I/isolation & purification, Histocompatibility Antigens Class II/immunology, Histocompatibility Antigens Class II/isolation & purification, Humans, Hybridomas, Neoplasms/immunology, Neoplasms/pathology, Peptides/immunology, Peptides/isolation & purification, Tandem Mass Spectrometry/instrumentation, Tandem Mass Spectrometry/methods, Time Factors, Affinity purification, Antigen processing and presentation, HLA class I and HLA class II, Human leukocyte antigen, Immunopeptidomics, Mass spectrometry
Pubmed
Create date
17/02/2019 15:24
Last modification date
10/10/2020 5:21
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