Regulation of the formin for3p by cdc42p and bud6p.

Details

Serval ID
serval:BIB_48AA3AAA46FB
Type
Article: article from journal or magazin.
Collection
Publications
Title
Regulation of the formin for3p by cdc42p and bud6p.
Journal
Molecular Biology of the Cell
Author(s)
Martin S.G., Rincón S.A., Basu R., Pérez P., Chang F.
ISSN
1059-1524 (Print)
ISSN-L
1059-1524
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
18
Number
10
Pages
4155-4167
Language
english
Abstract
Formins are conserved actin nucleators responsible for the assembly of diverse actin structures. Many formins are controlled through an autoinhibitory mechanism involving the interaction of a C-terminal DAD sequence with an N-terminal DID sequence. Here, we show that the fission yeast formin for3p, which mediates actin cable assembly and polarized cell growth, is regulated by a similar autoinhibitory mechanism in vivo. Multiple sites govern for3p localization to cell tips. The localization and activity of for3p are inhibited by an intramolecular interaction of divergent DAD and DID-like sequences. A for3p DAD mutant expressed at endogenous levels produces more robust actin cables, which appear to have normal organization and dynamics. We identify cdc42p as the primary Rho GTPase involved in actin cable assembly and for3p regulation. Both cdc42p, which binds at the N terminus of for3p, and bud6p, which binds near the C-terminal DAD-like sequence, are needed for for3p localization and full activity, but a mutation in the for3p DAD restores for3p localization and other phenotypes of cdc42 and bud6 mutants. In particular, the for3p DAD mutation suppresses the bipolar growth (NETO) defect of bud6Delta cells. These findings suggest that cdc42p and bud6p activate for3p by relieving autoinhibition.
Keywords
Amino Acid Sequence, Cell Cycle Proteins/chemistry, Cell Cycle Proteins/metabolism, Cell Shape, Microfilament Proteins/chemistry, Microfilament Proteins/metabolism, Molecular Sequence Data, Phenotype, Protein Structure, Tertiary, Protein Transport, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/metabolism, Sequence Deletion, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Create date
18/03/2008 11:32
Last modification date
20/08/2019 13:55
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