Protection from HIV-1 infection of primary CD4 T cells by CCR5 silencing is effective for the full spectrum of CCR5 expression.

Details

Serval ID
serval:BIB_488CC1E156F2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Protection from HIV-1 infection of primary CD4 T cells by CCR5 silencing is effective for the full spectrum of CCR5 expression.
Journal
Antiviral therapy
Author(s)
Butticaz C., Ciuffi A., Muñoz M., Thomas J., Bridge A., Pebernard S., Iggo R., Meylan P., Telenti A.
ISSN
1359-6535
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
8
Number
5
Pages
373-7
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
Stable gene silencing by RNA interference (RNAi) can be achieved by expression of small hairpin RNAs (shRNAs) from RNA polymerase III promoters. We have tested lentiviral vectors expressing shRNAs targetting CCR5 in primary CD4 T cells from donors representing various CCR5 and CCR2 genetic backgrounds covering the full spectrum of CCR5 expression levels and permissiveness for HIV-1 infection. A linear decrease in CCR5 expression resulted in a logarithmic decrease in cellular infection, giving up to three logs protection from HIV-1 infection in vitro. Protection was maintained at very high multiplicity of infection. This and other recent reports on RNAi should open a debate about the use of RNAi gene therapy for HIV infection.
Keywords
CD4-Positive T-Lymphocytes, Cells, Cultured, Gene Silencing, Gene Therapy, Genetic Vectors, HIV Infections, HIV-1, Humans, Lentivirus, RNA Interference, RNA, Small Interfering, Receptors, CCR5
Pubmed
Web of science
Create date
25/01/2008 14:45
Last modification date
20/08/2019 13:55
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