Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix®.

Details

Serval ID
serval:BIB_47DF56D93571
Type
Article: article from journal or magazin.
Collection
Publications
Title
Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix®.
Journal
Journal of Biomedical Optics
Author(s)
Martoccia C., Zellweger M., Lovisa B., Jichlinski P., van den Bergh H., Wagnières G.
ISSN
1560-2281 (Electronic)
ISSN-L
1083-3668
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
19
Number
9
Pages
97002
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
Fluorescence cystoscopy enhances detection of early bladder cancer. Water used to inflate the bladder during the procedure rapidly contains urine, which may contain fluorochromes. This frequently degradesfluorescence images. Samples of bladder washout fluid (BWF) or urine were collected (15 subjects). We studiedtheir fluorescence properties and assessed changes induced by pH (4 to 9) and temperature (15°C to 41°C).A typical fluorescence spectrum of BWF features a main peak (excitation/emission: 320∕420 nm, FWHM =50∕100 nm) and a weaker (5% to 20% of main peak intensity), secondary peak (excitation/emission: 455∕525 nm, FWHM = 80∕50 nm). Interpatient fluctuations of fluorescence intensity are observed. Fluorescence intensity decreases when temperature increases (max 30%) or pH values vary (max 25%). Neither approach is compatible with clinical settings. Fluorescence lifetime measurements suggest that 4-pyridoxic acid/riboflavin is the most likely molecule responsible for urine's main/secondary fluorescence peak. Our measurements give an insight into the spectroscopy of the detrimental background fluorescence. This should be included in the optical design of fluorescence cystoscopes. We estimate that restricting the excitation range from 370-430 nm to 395-415 nm would reduce the BWF background by a factor 2.
Pubmed
Web of science
Create date
27/11/2014 19:27
Last modification date
03/03/2018 16:51
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