Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea).

Details

Serval ID
serval:BIB_478559A7DE8D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea).
Journal
Molecular Ecology Resources
Author(s)
Yannic G., Sermier R., Aebischer A., Gavrilo M.V., Gilg O., Miljeteig C., Sabard B., Strøm H., Pouivé E., Broquet T.
ISSN
1755-0998 (Electronic)
ISSN-L
1755-098X
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
11
Number
5
Pages
877-889
Language
english
Abstract
We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n=21) and Russia (Severnaya Zemlya Archipelago, n=21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy-Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull.
Keywords
Alleles, Animals, Charadriiformes/genetics, Feathers/chemistry, Genetic Variation, Genotype, Greenland, Linkage Disequilibrium, Microsatellite Repeats/genetics, Mouth Mucosa/chemistry, Multiplex Polymerase Chain Reaction/methods, Russia
Pubmed
Web of science
Create date
04/05/2011 14:09
Last modification date
20/08/2019 13:53
Usage data