The mouse brain transcriptome by SAGE: differences in gene expression between P30 brains of the partial trisomy 16 mouse model of Down syndrome (Ts65Dn) and normals

Details

Serval ID
serval:BIB_4767E953CD1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The mouse brain transcriptome by SAGE: differences in gene expression between P30 brains of the partial trisomy 16 mouse model of Down syndrome (Ts65Dn) and normals
Journal
Genome Research
Author(s)
Chrast  R., Scott  H. S., Papasavvas  M. P., Rossier  C., Antonarakis  E. S., Barras  C., Davisson  M. T., Schmidt  C., Estivill  X., Dierssen  M., Pritchard  M., Antonarakis  S. E.
ISSN
1088-9051 (Print)
Publication state
Published
Issued date
12/2000
Volume
10
Number
12
Pages
2006-21
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
Trisomy 21, or Down syndrome (DS), is the most common genetic cause of mental retardation. Changes in the neuropathology, neurochemistry, neurophysiology, and neuropharmacology of DS patients' brains indicate that there is probably abnormal development and maintenance of central nervous system structure and function. The segmental trisomy mouse (Ts65Dn) is a model of DS that shows analogous neurobehavioral defects. We have studied the global gene expression profiles of normal and Ts65Dn male and normal female mice brains (P30) using the serial analysis of gene expression (SAGE) technique. From the combined sample we collected a total of 152,791 RNA tags and observed 45,856 unique tags in the mouse brain transcriptome. There are 14 ribosomal protein genes (nine under expressed) among the 330 statistically significant differences between normal male and Ts65Dn male brains, which possibly implies abnormal ribosomal biogenesis in the development and maintenance of DS phenotypes. This study contributes to the establishment of a mouse brain transcriptome and provides the first overall analysis of the differences in gene expression in aneuploid versus normal mammalian brain cells.
Keywords
Animals Blotting, Northern Brain Chemistry/*genetics Crosses, Genetic Disease Models, Animal Down Syndrome/*genetics Female Gene Expression Profiling/*methods Gene Library Genetic Markers/genetics Male Mice Mice, Inbred C3H Mice, Inbred C57BL Sequence Tagged Sites *Transcription, Genetic Trisomy/genetics
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:12
Last modification date
20/08/2019 13:53
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