Interleukin-12 production by human alveolar macrophages is controlled by the autocrine production of interleukin-10.

Details

Serval ID
serval:BIB_4745ECFA1F05
Type
Article: article from journal or magazin.
Collection
Publications
Title
Interleukin-12 production by human alveolar macrophages is controlled by the autocrine production of interleukin-10.
Journal
American Journal of Respiratory Cell and Molecular Biology
Author(s)
Isler P., de Rochemonteix B.G., Songeon F., Boehringer N., Nicod L.P.
ISSN
1044-1549[print], 1044-1549[linking]
Publication state
Published
Issued date
1999
Volume
20
Number
2
Pages
270-278
Language
english
Abstract
By releasing interleukin (IL)-12 in the lung, alveolar macrophages (AM) may profoundly modify an immune response. The autocrine regulation of the heterodimeric, biologically active form of IL-12 (IL-12 p70) by IL-10 was studied, as well as the expression of its subunits of 35 kD (p35) and 40 kD (p40). AM cultured in medium alone expressed only p35 mRNA. Both p35 and p40 mRNA levels were induced by lipopolysaccharide (LPS) and were further increased by interferon-gamma (IFN-gamma). LPS alone induced IL-12 p40 but not IL-12 p70 production in monocytes and in AM. However, IL-12 p70 was released when the autocrine production of IL-10 was neutralized by IL-10 blocking antibody, and IL-12 p40 production increased. Although IFN-gamma markedly decreased LPS-induced IL-10 production in AM, neutralizing IL-10 further enhanced the level of LPS and IFN-gamma-induced IL-12 p70 in AM. In contrast, neutralizing the trace amount of IL-10 released by AM stimulated by CD40 crosslinking and IFN-gamma did not increase IL-12 p70. Thus, IL-12 p70 production by AM appears to be tightly controlled by the autocrine release of IL-10 when stimulated by LPS, or by LPS and IFN-gamma, whereas CD40 crosslinking triggered IL-12 p70 production in the absence of autocrine regulation by IL-10.
Keywords
Antigens, CD40/pharmacology, Base Sequence, DNA Primers, Humans, Interferon-gamma/pharmacology, Interleukin-10/antagonists & inhibitors, Interleukin-10/biosynthesis, Interleukin-12/biosynthesis, Kinetics, Lipopolysaccharides/pharmacology, Macrophage Activation/drug effects, Macrophages, Alveolar/drug effects, Macrophages, Alveolar/metabolism
Pubmed
Web of science
Create date
19/02/2010 18:47
Last modification date
20/08/2019 13:53
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