In vivo effect of murine recombinant interleukin 3 on early hemopoietic progenitors.

Details

Serval ID
serval:BIB_46E5DC47230C
Type
Article: article from journal or magazin.
Collection
Publications
Title
In vivo effect of murine recombinant interleukin 3 on early hemopoietic progenitors.
Journal
European Journal of Immunology
Author(s)
Kindler V., Thorens B., Vassalli P.
ISSN
0014-2980[print], 0014-2980[linking]
Publication state
Published
Issued date
10/1987
Volume
17
Number
10
Pages
1511-1514
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Normal and irradiated mice were perfused with recombinant interleukin 3 (rIL3) and the number of early hemopoietic progenitors (CFU-S) was quantified in different organs. Normal mice perfused with rIL3 for 3 or 7 days showed a dramatic increase in the number of CFU-S in the spleen, liver and blood, while the bone marrow CFU-S number was slightly decreased. The total number of CFU-S per animal was only slightly increased; the major effect of rIL3 perfusion was thus on the distribution of CFU-S in the mice. Lethally irradiated mice injected with 5 X 10(4) syngeneic bone marrow cells and perfused with rIL3 were killed after 10 days. A significant increase in both the number and size of the splenic colonies was observed. The CFU-S content of these colonies, determined in a second set of irradiated mice, was increased by more than 25-fold, indicating that in conditions of hemopoietic depletion, rIL3 vastly increased the self-renewal capacity of early progenitors.
Keywords
Animals, Blood Cell Count, Bone Marrow/radiation effects, Hematopoietic Stem Cells/drug effects, Interleukin-3/pharmacology, Mice, Recombinant Proteins/pharmacology
Pubmed
Web of science
Create date
24/01/2008 14:41
Last modification date
03/03/2018 16:48
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