Specific processing of tenascin-C by the metalloprotease meprin beta neutralizes its inhibition of cell spreading

Details

Serval ID
serval:BIB_468B660C632D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Specific processing of tenascin-C by the metalloprotease meprin beta neutralizes its inhibition of cell spreading
Journal
Matrix Biology
Author(s)
Ambort Daniel, Brellier Florence, Becker-Pauly Christoph, Stoecker Walter, Andrejevic-Blant Snezana, Chiquet Matthias, Sterchi Erwin E.
ISSN
0945-053X
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
29
Number
1
Pages
31-42
Language
english
Abstract
The metalloprotease meprin has been implicated in tissue remodelling due to its capability to degrade extracellular matrix components. Here, we investigated the susceptibility of tenascin-C to cleavage by meprin beta and the functional properties of its proteolytic fragments. A set of monoclonal antibodies against chicken and human tenascin-C allowed the mapping of proteolytic fragments generated by meprin beta. In chicken tenascin-C, meprin beta processed all three major splicing variants by removal of 10 kDa N-terminal and 38 kDa C-terminal peptides, leaving a large central part of subunits intact. IN similar cleavage pattern was found for large human tenascin-C variant where two N-terminal peptides (10 or 15 kDa) and two C-terminal fragments (40 and 55 kDa) were removed from the intact subunit. N-terminal sequencing revealed the exact amino acid positions of cleavage sites. In both chicken and human tenascin-C N-terminal cleavages occurred just before and/or after the heptad repeats involved in subunit oligomerization. In the human protein, an additional cleavage site was identified in the alternative fibronectin type III repeat D. Whereas all these sites are known to be attacked by several other proteases, a unique cleavage by meprin beta was located to the 7th constant fibronectin type III repeat in both chicken and human tenascin-C, thereby removing the C-terminal domain involved in its anti-adhesive activity. In cell adhesion assays meprin beta-digested human tenascin-C was not able to interfere with fibronectin-mediated cell spreading, confirming cleavage in the anti-adhesive domain. Whereas the expression of meprin beta and tenascin-C does not overlap in normal colon tissue, inflamed lesions of the mucosa from patients with Crohn's disease exhibited many meprin beta-positive leukocytes in regions where tenascin-C was strongly induced. Our data indicate that, at least under pathological conditions, meprin beta might attack specific functional sites in tenascin-C that are important for its oligomerization and anti-adhesive activity. (C) 2009 Elsevier B.V. All rights reserved.
Keywords
Anti-Adhesive Activity, Antibody Mapping, Extracellular Matrix, Meprin Beta, N-Terminal Sequencing, Tenascin-C, Aminobenzoic Acid Hydrolase, Extracellular-Matrix, Alpha-Subunit, Rat-Kidney, In-Vitro, Differential Expression, Polyacrylamide Gels, Colorectal-Cancer, Focal Adhesion, Astacin Family
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Create date
11/03/2010 17:57
Last modification date
03/03/2018 16:48
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