Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma.

Details

Serval ID
serval:BIB_4680A78D988E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma.
Journal
The Journal of comparative neurology
Author(s)
Pasquettaz R., Kolotuev I., Rohrbach A., Gouelle C., Pellerin L., Langlet F.
ISSN
1096-9861 (Electronic)
ISSN-L
0021-9967
Publication state
Published
Issued date
08/06/2020
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Tanycytes are highly specialized ependymal cells that line the bottom and the lateral walls of the third ventricle. In contact with the cerebrospinal fluid through their cell bodies, they send processes into the arcuate nucleus, the ventromedial nucleus, and the dorsomedial nucleus of the hypothalamus. In the present work, we combined transgenic and immunohistochemical approaches to investigate the neuroanatomical associations between tanycytes and neural cells present in the hypothalamic parenchyma, in particular in the arcuate nucleus. The specific expression of tdTomato in tanycytes first allowed the observation of peculiar subcellular protrusions along tanycyte processes and at their endfeet such as spines, swelling, en passant boutons, boutons, or claws. Interestingly, these protrusions contact different neural cells in the brain parenchyma including blood vessels and neurons, and in particular NPY and POMC neurons in the arcuate nucleus. Using both fluorescent and electron microscopy, we finally observed that these tanycyte protrusions contain ribosomes, mitochondria, diverse vesicles, and transporters, suggesting dense tanycyte/neuron and tanycyte/blood vessel communications. Altogether, our results lay the neuroanatomical basis for tanycyte/neural cell interactions, which will be useful to further understand cell-to-cell communications involved in the regulation of neuroendocrine functions.
Keywords
Arcuate neurons, Electron microscopy, Fluorescence microscopy, Glia-Neuron communication, Hypothalamus, Tanycytes, arcuate neurons, electron microscopy, fluorescence microscopy, glia-neuron communication, hypothalamus, tanycytes
Pubmed
Web of science
Open Access
Yes
Create date
10/06/2020 14:41
Last modification date
31/07/2020 5:26
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