Free Fatty Acids Impair FGF21 Action in HepG2 Cells.

Details

Serval ID
serval:BIB_461A22BDA986
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Free Fatty Acids Impair FGF21 Action in HepG2 Cells.
Journal
Cellular Physiology and Biochemistry
Author(s)
Asrih M., Montessuit C., Philippe J., Jornayvaz F.R.
ISSN
1421-9778 (Electronic)
ISSN-L
1015-8987
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
37
Number
5
Pages
1767-1778
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
BACKGROUND/AIMS: Fibroblast growth factor 21 (FGF21) is a key mediator of glucose and lipid metabolism. However, the beneficial effects of exogenous FGF21 administration are attenuated in obese animals and humans with elevated levels of circulating free fatty acids (FFA).
METHODS: We investigated in vitro how FFA impact FGF21 effects on hepatic lipid metabolism.
RESULTS: In the absence of FFA, FGF21 reduced lipogenesis and increased lipid oxidation in HepG2 cells. Inhibition of lipogenesis was associated with a down regulation of SREBP-1c, FAS and SCD1. The lipid-lowering effect was associated with AMPK and ACC phosphorylation, and up regulation of CPT-1α expression. Further, FGF21 treatment reduced TNFα gene expression, suggesting a beneficial action of FGF21 on inflammation. In contrast, the addition of FFA abolished the positive effects of FGF21 on lipid metabolism.
CONCLUSION: In the absence of FFA, FGF21 improves lipid metabolism in HepG2 cells and reduces the inflammatory cytokine TNFα. However, under high levels of FFA, FGF21 action on lipid metabolism and TNFα gene expression is impaired. Therefore, FFA impair FGF21 action in HepG2 cells potentially through TNFα.
Pubmed
Web of science
Open Access
Yes
Create date
03/01/2016 16:51
Last modification date
20/08/2019 13:51
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