Arbuscular mycorrhizal fungi (Glomeromycota) harbour ancient fungal tubulin genes that resemble those of the chytrids (Chytridiomycota).

Details

Ressource 1Download: BIB_459678F4DD50.P001.pdf (543.33 [Ko])
State: Serval
Version: author
Serval ID
serval:BIB_459678F4DD50
Type
Article: article from journal or magazin.
Collection
Publications
Title
Arbuscular mycorrhizal fungi (Glomeromycota) harbour ancient fungal tubulin genes that resemble those of the chytrids (Chytridiomycota).
Journal
Fungal Genetics and Biology
Author(s)
Corradi N., Hijri M., Fumagalli L., Sanders I.R.
ISSN
1087-1845[print], 1087-1845[linking]
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
41
Number
11
Pages
1037-1045
Language
english
Abstract
The genes encoding alpha- and beta-tubulins have been widely sampled in most major fungal phyla and they are useful tools for fungal phylogeny. Here, we report the first isolation of alpha-tubulin sequences from arbuscular mycorrhizal fungi (AMF). In parallel, AMF beta-tubulins were sampled and analysed to identify the presence of paralogs of this gene. The AMF alpha-tubulin amino acid phylogeny was congruent with the results previously reported for AMF beta-tubulins and showed that AMF tubulins group together at a basal position in the fungal clade and showed high sequence similarities with members of the Chytridiomycota. This is in contrast with phylogenies for other regions of the AMF genome. The amount and nature of substitutions are consistent with an ancient divergence of both orthologs and paralogs of AMF tubulins. At the amino acid level, however, AMF tubulins have hardly evolved from those of the chytrids. This is remarkable given that these two groups are ancient and the monophyletic Glomeromycota probably diverged from basal fungal ancestors at least 500 million years ago. The specific primers we designed for the AMF tubulins, together with the high molecular variation we found among the AMF species we analysed, make AMF tubulin sequences potentially useful for AMF identification purposes.
Keywords
Base Sequence, DNA Primers, DNA, Fungal/genetics, DNA, Fungal/isolation & purification, Fungi/classification, Fungi/genetics, Phylogeny, Tubulin/genetics
Pubmed
Web of science
Create date
24/01/2008 20:09
Last modification date
03/03/2018 16:46
Usage data