Organization of double-stranded DNA in bacteriophages: a study by cryo-electron microscopy of vitrified samples

Details

Serval ID
serval:BIB_456ECABDCD17
Type
Article: article from journal or magazin.
Collection
Publications
Title
Organization of double-stranded DNA in bacteriophages: a study by cryo-electron microscopy of vitrified samples
Journal
EMBO Journal
Author(s)
Lepault  J., Dubochet  J., Baschong  W., Kellenberger  E.
ISSN
0261-4189 (Print)
Publication state
Published
Issued date
05/1987
Volume
6
Number
5
Pages
1507-1512
Notes
Journal Article --- Old month value: May
Abstract
In this paper it is shown that conformation and packing of double-stranded DNA within the head of bacteriophages lambda and T4 can be assessed by cryo-electron microscopy of vitrified specimens. Electron diffraction patterns show that DNA within vitrified bacteriophages has a B conformation. Electron micrographs of vitrified bacteriophages show domains within the head formed by a approximately 2.5-nm striation and arising from the DNA packing. The number of differently oriented domains seen within a vitrified bacteriophage depends upon the geometry of the DNA container: the bacteriophage capsid. The packing of DNA within bacteriophages seems then to be governed by at least two phenomena. The first is the tendency of DNA to form local alignments (nematic liquid crystals). The second is the orientation of these liquid crystals by the bacteriophage capsid. From these observations we propose a possible packaging mechanism: constrained nematic crystallization.
Keywords
Bacteriophage lambda/genetics/*ultrastructure DNA, Viral/*ultrastructure Escherichia coli/*ultrastructure *Genes, Viral Microscopy, Electron Nucleic Acid Conformation T-Phages/genetics/*ultrastructure
Pubmed
Web of science
Create date
24/01/2008 11:25
Last modification date
03/03/2018 16:45
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