Is pain temporary and glory forever? Detection of tramadol using dried blood spot in cycling competitions.

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_4555B9A3BC71
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Is pain temporary and glory forever? Detection of tramadol using dried blood spot in cycling competitions.
Journal
Drug testing and analysis
Author(s)
Salamin O., Garcia A., González-Ruiz V., Rossi F., Bigard X., Déglon J., Daali Y., Faiss R., Saugy M., Rudaz S.
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Publication state
Published
Issued date
11/2020
Peer-reviewed
Oui
Volume
12
Number
11-12
Pages
1649-1657
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Tramadol is a synthetic opioid drug used in the treatment of chronic and acute pain. An abnormal prevalence of its misuse in elite sport to overcome pain resulting from prolonged physical effort was recently reported. However, besides its antinociceptive effects, tramadol consumption is associated with negative effects such as numbness, confusion, and reduced alertness. This fact prompted the Union Cycliste Internationale to ban the use of tramadol in cycling competitions. Herein, we present the development of a dried blood spot (DBS) sample collection and preparation method followed by a liquid-chromatography mass spectrometry (LC-MS) analysis to rapidly determine the presence of tramadol and its two main metabolites in blood samples. The detection window of each analyte was evaluated and the analysis of performance on various MS platforms (HRMS and MS/MS) was assessed. Tramadol and its two main metabolites were detected up to 12 h after the intake of a single dose of 50 mg of tramadol in positive controls. In professional cycling competitions, 711 DBS samples collected from 361 different riders were analysed using the developed methodology, but all returned negative results (absence of parent and both metabolite compounds). In the context of professional cycling, we illustrate a valid method bringing together the easiness of collection and minimal sample preparation required by DBS, yet affording the performance standards of MS determination. The proposed method to detect tramadol and its metabolites was successfully implemented in cycling races with a probable strong deterrent effect.
Keywords
DBS, UHPLC-MS/MS, cycling, metabolites, tramadol
Pubmed
Web of science
Create date
09/09/2020 11:42
Last modification date
23/02/2021 7:26
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