Mapping and comparison of the B-cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys

Details

Serval ID
serval:BIB_4543F7333940
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mapping and comparison of the B-cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys
Journal
Annals of Tropical Medicine and Parasitology
Author(s)
Arevalo-Herrera  M., Roggero  M. A., Gonzalez  J. M., Vergara  J., Corradin  G., Lopez  J. A., Herrera  S.
ISSN
0003-4983 (Print)
Publication state
Published
Issued date
07/1998
Volume
92
Number
5
Pages
539-51
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Abstract
Plasma samples of individuals from two malaria-endemic villages on the Colombian Pacific coast and synthetic peptides representing different fragments of the central and flanking regions of the Plasmodium vivax circumsporozoite protein (CSP) were used to perform a fine mapping of the B-cell epitopes on the whole CSP. In addition, the immunogenicity of long polypeptides corresponding to the amino (N) and carboxyl (C) regions was evaluated in Aotus monkeys. The epitopes recognized after natural infection of humans and after immunization of Aotus with these synthetic peptides were compared. Human samples more frequently contained specific antibodies to the central region. The type-I repeat region of the CSP was predominantly recognized by the human sera (by 68% of those from the village of Zacarias and 75% of those from Bajo Calima), a significantly smaller population reacting with the type-II repeat (20% and 11%, respectively). Most of the sera reacting with the type-I repeat recognized the minimal epitope AGDR. Although the N- and C-terminal polypeptides were both highly immunogenic in Aotus and induced long-lasting antibodies, titres of antibodies to the C-terminal polypeptide were higher than those of antibodies to the N-terminal. Competitive inhibition assays performed using human and monkey plasma allowed the identification of dominant B-cell epitopes on sequence 71-90 (p8) from the amino region and sequence 332-361 (p24/p25) from the carboxyl region. The high prevalence of naturally induced antibodies to the three epitopes, the possible functional role of the corresponding sequences, and the high immunogenicity of these epitopes in Aotus could be of great importance in the development of a malaria vaccine based on P. vivax CSP.
Keywords
Adult Aged Aged, 80 and over Animals Antibodies, Protozoan/analysis Aotidae B-Lymphocytes/*immunology Colombia Enzyme-Linked Immunosorbent Assay Epitope Mapping Epitopes/*analysis Female Humans Lymphocyte Activation Malaria, Vivax/immunology Male Middle Aged Plasmodium vivax/*immunology Protozoan Proteins/*immunology
Pubmed
Web of science
Create date
24/01/2008 15:55
Last modification date
03/03/2018 16:45
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