Cx36 preferentially connects beta-cells within pancreatic islets

Details

Serval ID
serval:BIB_452ABB97CF0A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cx36 preferentially connects beta-cells within pancreatic islets
Journal
Diabetes
Author(s)
Serre-Beinier  V., Le Gurun  S., Belluardo  N., Trovato-Salinaro  A., Charollais  A., Haefliger  J. A., Condorelli  D. F., Meda  P.
ISSN
0012-1797 (Print)
Publication state
Published
Issued date
05/2000
Volume
49
Number
5
Pages
727-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Previous studies have provided evidence for the transcripts of Cx43 and Cx45 within pancreatic islets. As of yet, however, it has proven difficult to unambiguously demonstrate the expression of these proteins by islet cells. We have investigated whether Cx36, a new connexin species recently identified in mammalian brain and retina, may also be expressed in pancreatic islets. Using probes that permitted the original identification of Cx36 in the central nervous system, we show that a transcript for Cx36 is clearly detectable in rat pancreatic islets. Using novel and affinity-purified polyclonal antibodies, we have found that Cx36 is actually expressed in pancreatic islets. Both in situ hybridization and immunolabeling indicated that this connexin is abundant in the centrally located insulin-producing beta-cells and is expressed much less, if at all, by the other endocrine cell types. This differential expression was further confirmed on fluorescence-activated cell sorter-purified preparations enriched in either beta- or non-beta-cells. The finding of a differential distribution of Cx36 within distinct regions of pancreatic islets creates the possibility that this connexin may provide the establishment of selective pathways of communication between the different types of endocrine cells comprising the pancreatic islet.
Keywords
Animals Connexins/genetics/*metabolism Eye Proteins/genetics/*metabolism Immunologic Techniques In Situ Hybridization Insulin/biosynthesis Islets of Langerhans/*metabolism RNA, Messenger/metabolism Rats Rats, Inbred Strains Reverse Transcriptase Polymerase Chain Reaction Tissue Distribution
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:48
Last modification date
20/08/2019 14:49
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