Seasonal fluctuations of bacterial community diversity in agricultural soil and experimental validation by laboratory disturbance experiments.

Details

Serval ID
serval:BIB_44B11AA49069
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Seasonal fluctuations of bacterial community diversity in agricultural soil and experimental validation by laboratory disturbance experiments.
Journal
Microbial Ecology
Author(s)
Meier C., Wehrli B., van der Meer J.R.
ISSN
0095-3628[print], 0095-3628[linking]
Publication state
Published
Issued date
2008
Volume
56
Number
2
Pages
210-222
Language
english
Abstract
Natural fluctuations in soil microbial communities are poorly documented because of the inherent difficulty to perform a simultaneous analysis of the relative abundances of multiple populations over a long time period. Yet, it is important to understand the magnitudes of community composition variability as a function of natural influences (e.g., temperature, plant growth, or rainfall) because this forms the reference or baseline against which external disturbances (e.g., anthropogenic emissions) can be judged. Second, definition of baseline fluctuations in complex microbial communities may help to understand at which point the systems become unbalanced and cannot return to their original composition. In this paper, we examined the seasonal fluctuations in the bacterial community of an agricultural soil used for regular plant crop production by using terminal restriction fragment length polymorphism profiling (T-RFLP) of the amplified 16S ribosomal ribonucleic acid (rRNA) gene diversity. Cluster and statistical analysis of T-RFLP data showed that soil bacterial communities fluctuated very little during the seasons (similarity indices between 0.835 and 0.997) with insignificant variations in 16S rRNA gene richness and diversity indices. Despite overall insignificant fluctuations, between 8 and 30% of all terminal restriction fragments changed their relative intensity in a significant manner among consecutive time samples. To determine the magnitude of community variations induced by external factors, soil samples were subjected to either inoculation with a pure bacterial culture, addition of the herbicide mecoprop, or addition of nutrients. All treatments resulted in statistically measurable changes of T-RFLP profiles of the communities. Addition of nutrients or bacteria plus mecoprop resulted in bacteria composition, which did not return to the original profile within 14 days. We propose that at less than 70% similarity in T-RFLP, the bacterial communities risk to drift apart to inherently different states.
Keywords
2-Methyl-4-chlorophenoxyacetic Acid/analogs & derivatives, 2-Methyl-4-chlorophenoxyacetic Acid/pharmacology, Agriculture, Bacteria/classification, Bacteria/drug effects, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Ecosystem, Genes, rRNA, Herbicides/pharmacology, Laboratories, Polymorphism, Restriction Fragment Length, Principal Component Analysis, RNA, Ribosomal, 16S/genetics, Seasons, Sequence Analysis, DNA, Soil/analysis, Soil Microbiology
Pubmed
Web of science
Create date
01/02/2008 14:01
Last modification date
20/08/2019 14:49
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