Dexamethasone selectively regulates the activity of enzymatic markers of cerebral endothelial cell lines.

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_4484CB45DA09
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dexamethasone selectively regulates the activity of enzymatic markers of cerebral endothelial cell lines.
Journal
In vitro cellular & developmental biology
Author(s)
Juillerat-Jeanneret L., Aguzzi A., Wiestler O.D., Darekar P., Janzer R.C.
ISSN
0883-8364 (Print)
ISSN-L
0883-8364
Publication state
Published
Issued date
1992
Peer-reviewed
Oui
Volume
28A
Number
7-8
Pages
537-543
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Two endothelial cell lines were derived from grafts of the central nervous system using retrovirus mediated gene transfer to introduce the polyoma middle-T oncogene into fetal rat brain endothelial cells and transplantation of these cells into adult rat brain. In this report, we further characterize these cells and the effect of dexamethasone on the expression of specific enzymatic markers. These cells take up acetylated low density lipoprotein, leucine, and glucose, and express Factor VIII-related antigen, angiotensin converting enzyme, alkaline phosphatase, gamma-glutamyltranspeptidase, and as yet undescribed aminopeptidase A and B-like enzymes. When grown on semi-permeable membranes, these transformed cells do not spontaneously retain small hydrophilic molecules. In culture, one of the lines (EC 193) forms a confluent monolayer of spindle-shaped cells homogenously expressing gamma-glutamyltranspeptidase at a level comparable to primary cells. The other cell line (EC 219) grows as clusters of elongated cells, and gamma-glutamyltranspeptidase activity is expressed mainly in cells forming the clusters. This clustered pattern changes to a confluent one after culture on type-I collagen. Dexamethasone increases angiotensin-converting enzyme activity, and decreases the expression of gamma-glutamyltranspeptidase and aminopeptidase A, whereas the aminopeptidase B activity is little modified. Inhibition of aminopeptidase A activity by amastatin, potentiates angiotensin II effects on DNA synthesis. These results indicate that retrovirally transformed brain endothelial cells are a useful model for studying the blood-brain barrier in vitro and that dexamethasone, an agent with the potential to reduce brain edema, directly affects some blood-brain barrier properties in these endothelial cell lines.
Keywords
Aminopeptidases/antagonists & inhibitors, Aminopeptidases/metabolism, Animals, Anti-Bacterial Agents, Blood-Brain Barrier/physiology, Brain/blood supply, Cells, Cultured/drug effects, Dexamethasone/pharmacology, Endothelium, Vascular/drug effects, Enzyme Activation/drug effects, Genetic Vectors, Glutamyl Aminopeptidase, Mice, Oligopeptides/pharmacology, Peptides, Peptidyl-Dipeptidase A/metabolism, Polyomavirus, Rats, Transfection, gamma-Glutamyltransferase/metabolism
Pubmed
Web of science
Create date
29/01/2008 19:33
Last modification date
16/07/2020 9:42
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