Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex.

Details

Serval ID
serval:BIB_43D695BDB590
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex.
Journal
Scientific reports
Author(s)
Bizzotto S., Uzquiano A., Dingli F., Ershov D., Houllier A., Arras G., Richards M., Loew D., Minc N., Croquelois A., Houdusse A., Francis F.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
11/12/2017
Peer-reviewed
Oui
Volume
7
Number
1
Pages
17308
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
The ventricular zone (VZ) of the developing cerebral cortex is a pseudostratified epithelium that contains progenitors undergoing precisely regulated divisions at its most apical side, the ventricular lining (VL). Mitotic perturbations can contribute to pathological mechanisms leading to cortical malformations. The HeCo mutant mouse exhibits subcortical band heterotopia (SBH), likely to be initiated by progenitor delamination from the VZ early during corticogenesis. The causes for this are however, currently unknown. Eml1, a microtubule (MT)-associated protein of the EMAP family, is impaired in these mice. We first show that MT dynamics are perturbed in mutant progenitor cells in vitro. These may influence interphase and mitotic MT mechanisms and indeed, centrosome and primary cilia were altered and spindles were found to be abnormally long in HeCo progenitors. Consistently, MT and spindle length regulators were identified in EML1 pulldowns from embryonic brain extracts. Finally, we found that mitotic cell shape is also abnormal in the mutant VZ. These previously unidentified VZ characteristics suggest altered cell constraints which may contribute to cell delamination.
Keywords
Animals, Cells, Cultured, Cerebral Cortex/metabolism, Cerebral Cortex/pathology, Classical Lissencephalies and Subcortical Band Heterotopias/metabolism, Classical Lissencephalies and Subcortical Band Heterotopias/pathology, Female, Mice, Mice, Knockout, Microtubule-Associated Proteins/physiology, Neural Stem Cells/metabolism, Neural Stem Cells/pathology, Spindle Apparatus/metabolism, Spindle Apparatus/pathology
Pubmed
Web of science
Open Access
Yes
Create date
14/12/2017 18:35
Last modification date
20/08/2019 14:47
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