Interactions of processed Nef (58-206) with virion proteins of HIV type 1

Details

Serval ID
serval:BIB_43A333F54B1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interactions of processed Nef (58-206) with virion proteins of HIV type 1
Journal
AIDS Research and Human Retroviruses
Author(s)
Ciuffi  A., Munoz  M., Bleiber  G., Favre  M., Stutz  F., Telenti  A., Meylan  P. R.
ISSN
0889-2229 (Print)
Publication state
Published
Issued date
04/2004
Volume
20
Number
4
Pages
399-407
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
The Nef protein plays a major role in vivo in promoting HIV and SIV replication and pathogenesis. In vitro, Nef has been shown to down-regulate cell surface molecules, such as CD4 and MHC-I, alter T cell signaling, and enhance virion infectivity. These effects are attributed to interactions of Nef with cellular proteins. In addition, HIV Nef is incorporated into viral particles, mainly localizing in the virion cores. However, no report has been published to date regarding Nef interactions with virion proteins. By immunoprecipitation, Nef was found to bind to viral enzymes. Using yeast two-hybrid and GST pulldown procedures to find out direct potential partners of Nef, Nef was consistently found to interact with viral integrase (IN). The interaction between Nef and IN was stronger when Nef was present as the viral protease-cleaved isoform. We hypothesize that the interaction of Nef with viral integrase or other virion proteins may explain the presence of Nef in viral cores. In addition, this interaction suggests that Nef may accompany the reverse transcription and the preintegration complexes during the early steps of the infection cycle and potentially affect infectivity during these steps.
Keywords
Gene Products, nef/chemistry/*metabolism HIV Integrase/metabolism HIV Protease/metabolism HIV-1/*metabolism HIV-1 Reverse Transcriptase/metabolism Protein Binding Two-Hybrid System Techniques Viral Core Proteins/chemistry/*metabolism Virion/metabolism
Pubmed
Web of science
Create date
25/01/2008 15:46
Last modification date
20/08/2019 14:47
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