Heterogeneity of Microvascular Endothelial Cells of the Brain: A Comparison of the Effects of Extracellular Matrix and Soluble Astrocytic Factors

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State: Public
Version: Final published version
License: All rights reserved
Serval ID
serval:BIB_435813972BAD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneity of Microvascular Endothelial Cells of the Brain: A Comparison of the Effects of Extracellular Matrix and Soluble Astrocytic Factors
Journal
Endothelium
Author(s)
Juillerat-Jeanneret L., Darekar P., Janzer R. C.
ISSN
1062-3329
Publication state
Published
Issued date
1993
Peer-reviewed
Oui
Volume
1
Number
2
Pages
85-91
Language
english
Abstract
Until recently, microvascular endothelial cells of the blood-brain barrier were considered a homogeneous population, whose properties were induced by astrocytes. To address the question of brain endothelial cell heterogeneity, we measured the activity of specific markers of endothelial cells of different origins, including two brain-derived endothelial cell lines grown on plastic or on extracellular matrix (ECM) components secreted either by a glial or a fibroblast cell line, with or without the addition of astrocyte-conditioned medium (ACM). ECM components and ACM conjointly, but differently in each endothelial cell line, increased the uptake of leucine and modulated the expression of the specific markers alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGTP) in both cerebral endothelial lines. ACM decreased the incorporation of thymidine in the pulmonary endothelial cell line, but not in a cerebral cell line. An induction of blood-brain barrier specific GGTP was never observed in endothelial cells of non-cerebral origin. These results demonstrate that subpopulations of cerebral endothelial cells exist, that astrocyte-derived soluble factors and ECM components can modulate the expression and the function of markers of endothelial cells and that astrocyte-derived soluble factors have a selective growth inhibitory effect on endothelial cells of non-cerebral origin
Keywords
Astrocytes/Blood-Brain Barrier/Brain/Cell Line/Cells/Endothelial Cells/Extracellular Matrix
Create date
30/01/2008 18:10
Last modification date
14/07/2020 5:21
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