Loss of interleukin 2 dependence in cloned interleukin 2-dependent rat T lymphocyte x BW5147 hybridomas is not associated with segregation of a specific pair of rat chromosomes.

Details

Serval ID
serval:BIB_43429C1BE312
Type
Article: article from journal or magazin.
Collection
Publications
Title
Loss of interleukin 2 dependence in cloned interleukin 2-dependent rat T lymphocyte x BW5147 hybridomas is not associated with segregation of a specific pair of rat chromosomes.
Journal
European journal of immunology
Author(s)
Somoza C., Fernández-Ruiz E., Jotterand-Bellomo M., Sanz E., Nabholz M., Silva A.
ISSN
0014-2980
Publication state
Published
Issued date
1989
Volume
19
Number
7
Pages
1177-81
Language
english
Abstract
A fusion between the mouse AKR thymoma BW5147 and a culture of homogeneously OX8+ (CD8) rat T lymphoblasts yield interleukin (IL) 2-dependent T cell hybridomas when selected in HAT medium supplemented with IL 2-containing supernatants of concanvalin A-activated cells and dexamethasone. IL 2-independent variants can be selected from cloned IL2-dependent hybrids in the absence of conditioned medium. Karyotype analysis was used to test a previously proposed hypothesis according to which IL2-independent variants arise through loss of a specific cytotoxic T lymphocyte (rat) chromosome carrying a gene responsible for IL2 dependence. Comparison of karyotypes of several independently derived hybrids with those of their IL 2-independent variants showed that the hybrids contain at least one homologue of all rat chromosomes, and that no pair of rat chromosomes is consistently absent in the IL 2-independent variants.
Keywords
Animals, Cell Division, Chromosome Banding, Hybridomas, Interleukin-2, Mice, Phenotype, Rats, T-Lymphocytes
Pubmed
Create date
22/05/2009 8:56
Last modification date
20/08/2019 13:47
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