Separating the contribution of glucocorticoids and wakefulness to the molecular and electrophysiological correlates of sleep homeostasis.

Details

Serval ID
serval:BIB_424653092D75
Type
Article: article from journal or magazin.
Collection
Publications
Title
Separating the contribution of glucocorticoids and wakefulness to the molecular and electrophysiological correlates of sleep homeostasis.
Journal
Sleep
Author(s)
Mongrain V., Hernandez S.A., Pradervand S., Dorsaz S., Curie T., Hagiwara G., Gip P., Heller H.C., Franken P.
ISSN
0161-8105[print], 0161-8105[linking]
Publication state
Published
Issued date
2010
Volume
33
Number
9
Pages
1147-1157
Language
english
Abstract
Study Objectives: The sleep-deprivation-induced changes in delta power, an electroencephalographical correlate of sleep need, and brain transcriptome profiles have importantly contributed to current hypotheses on sleep function. Because sleep deprivation also induces stress, we here determined the contribution of the corticosterone component of the stress response to the electrophysiological and molecular markers of sleep need in mice.
Design: N/A
Settings: Mouse sleep facility.
Participants: C57BL/6J, AKR/J, DBA/2J mice.
Interventions: Sleep deprivation, adrenalectomy (ADX).
Measurements and Results: Sleep deprivation elevated corticosterone levels in 3 inbred strains, but this increase was larger in DBA/2J mice; i.e., the strain for which the rebound in delta power after sleep deprivation failed to reach significance. Elimination of the sleep-deprivation-associated corticosterone surge through ADX in DBA/2J mice did not, however, rescue the delta power rebound but did greatly reduce the number of transcripts affected by sleep deprivation. Genes no longer affected by sleep deprivation cover pathways previously implicated in sleep homeostasis, such as lipid, cholesterol (e.g., Ldlr, Hmgcs1, Dhcr7, -24, Fkbp5), energy and carbohydrate metabolism (e.g., Eno3, G6pc3, Mpdu1, Ugdh, Man1b1), protein biosynthesis (e.g., Sgk1, Alad, Fads3, Eif2c2, -3, Mat2a), and some circadian genes (Per1, -3), whereas others, such as Homer1a, remained unchanged. Moreover, several microRNAs were affected both by sleep deprivation and ADX.
Conclusions: Our findings indicate that corticosterone contributes to the sleep-deprivation-induced changes in brain transcriptome that have been attributed to wakefulness per se. The study identified 78 transcripts that respond to sleep loss independent of corticosterone and time of day, among which genes involved in neuroprotection prominently feature, pointing to a molecular pathway directly relevant for sleep function.
Keywords
Adrenalectomy, Animals, Circadian Rhythm Signaling Peptides and Proteins/genetics, Circadian Rhythm Signaling Peptides and Proteins/metabolism, Corticosterone/secretion, Disease Models, Animal, Electroencephalography, Genotype, Glucocorticoids/physiology, Homeostasis/physiology, Male, Mice, Mice, Inbred Strains, RNA, Messenger/metabolism, Sleep Deprivation/etiology, Sleep Deprivation/metabolism, Wakefulness/physiology
Pubmed
Web of science
Create date
10/03/2011 17:43
Last modification date
20/08/2019 14:44
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