Interlinked sister chromosomes arise in the absence of condensin during fast replication in B. subtilis.
Details
Serval ID
serval:BIB_41E90C2572DF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interlinked sister chromosomes arise in the absence of condensin during fast replication in B. subtilis.
Journal
Current Biology
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
24
Number
3
Pages
293-298
Language
english
Abstract
Condensin-an SMC-kleisin complex-is essential for efficient segregation of sister chromatids in eukaryotes [1-4]. In Escherichia coli and Bacillus subtilis, deletion of condensin subunits results in severe growth phenotypes and the accumulation of cells lacking nucleoids [5, 6]. In many other bacteria and under slow growth conditions, however, the reported phenotypes are much milder or virtually absent [7-10]. This raises the question of what role prokaryotic condensin might play during chromosome segregation under various growth conditions. In B. subtilis and Streptococcus pneumoniae, condensin complexes are enriched on the circular chromosome near the single origin of replication by ParB proteins bound to parS sequences [11, 12]. Using conditional alleles of condensin in B. subtilis, we demonstrate that depletion of its activity results in an immediate and severe defect in the partitioning of replication origins. Multiple copies of the chromosome remain unsegregated at or near the origin of replication. Surprisingly, the growth and chromosome segregation defects in rich medium are suppressed by a reduction of replication fork velocity but not by partial inhibition of translation or transcription. Prokaryotic condensin likely prevents the formation of sister DNA interconnections at the replication fork or promotes their resolution behind the fork.
Keywords
Adenosine Triphosphatases/metabolism, Bacillus subtilis/physiology, Bacterial Proteins/metabolism, Chromosome Segregation/physiology, Chromosomes, Bacterial/metabolism, DNA-Binding Proteins/metabolism, Multiprotein Complexes/metabolism, Replication Origin/physiology
Pubmed
Web of science
Open Access
Yes
Create date
17/08/2016 9:51
Last modification date
20/08/2019 13:43