Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins

Details

Serval ID
serval:BIB_4109611FD5EB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stable human lymphoblastoid cell lines constitutively expressing hepatitis C virus proteins
Journal
Journal of General Virology
Author(s)
Wolk  B., Gremion  C., Ivashkina  N., Engler  O. B., Grabscheid  B., Bieck  E., Blum  H. E., Cerny  A., Moradpour  D.
ISSN
0022-1317 (Print)
Publication state
Published
Issued date
06/2005
Volume
86
Number
Pt 6
Pages
1737-46
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
The cellular immune response plays a central role in virus clearance and pathogenesis of liver disease in hepatitis C. The study of hepatitis C virus (HCV)-specific immune responses is limited by currently available cell-culture systems. Here, the establishment and characterization of stable human HLA-A2-positive B-lymphoblastoid x T hybrid cell lines constitutively expressing either the NS3-4A complex or the entire HCV polyprotein are reported. These cell lines, termed T1/NS3-4A and T1/HCVcon, respectively, were maintained in continuous culture for more than 1 year with stable characteristics. HCV structural and non-structural proteins were processed accurately, indicating that the cellular and viral proteolytic machineries are functional in these cell lines. Viral proteins were found in the cytoplasm in dot-like structures when expressed in the context of the HCV polyprotein or in a perinuclear fringe when the NS3-4A complex was expressed alone. T1/NS3-4A and T1/HCVcon cells were lysed efficiently by HCV-specific cytotoxic T lymphocytes from patients with hepatitis C and from human HLA-A2.1 transgenic mice immunized with a liposomal HCV vaccine, indicating that viral proteins are processed endogenously and presented efficiently via the major histocompatibility complex class I pathway. In conclusion, these cell lines represent a unique tool to study the cellular immune response, as well as to evaluate novel vaccine and immunotherapeutic strategies against HCV.
Keywords
Animals Cell Line, Tumor/metabolism Clone Cells/metabolism Cytoplasm/metabolism HLA-A2 Antigen/genetics Hepacivirus/*metabolism Hepatitis C/immunology Mice Mice, Transgenic Polyproteins/metabolism Viral Hepatitis Vaccines/immunology Viral Nonstructural Proteins/*metabolism Viral Structural Proteins/*metabolism
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:05
Last modification date
20/08/2019 13:40
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