Clinical evaluation of a method for detecting superficial surgical transitional cell carcinoma of the bladder by light-induced fluorescence of protoporphyrin IX following the topical application of 5-aminolevulinic acid: preliminary results.

Details

Serval ID
serval:BIB_4103DCCC6568
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clinical evaluation of a method for detecting superficial surgical transitional cell carcinoma of the bladder by light-induced fluorescence of protoporphyrin IX following the topical application of 5-aminolevulinic acid: preliminary results.
Journal
Lasers in surgery and medicine
Author(s)
Jichlinski P., Forrer M., Mizeret J., Glanzmann T., Braichotte D., Wagnières G., Zimmer G., Guillou L., Schmidlin F., Graber P., van den Bergh H., Leisinger H.J.
ISSN
0196-8092
Publication state
Published
Issued date
1997
Peer-reviewed
Oui
Volume
20
Number
4
Pages
402-8
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
BACKGROUND AND OBJECTIVE: In bladder cancer, conventional white light endoscopic examination of the bladder does not provide adequate information about the presence of "flat" urothelial lesions such as carcinoma in situ. In the present investigation, we examine a new technique for the photodetection of such lesions by the imaging of protoporphyrin IX (PpIX) fluorescence following topical application of 5-aminolevulinic acid (ALA). STUDY DESIGN/MATERIALS AND METHODS: Several hours after bladder instillation of an aqueous solution of ALA in 34 patients, a Krypton ion laser or a filtered Xenon arc-lamp was used to excite PpIX fluorescence. Tissue samples for histological analysis were taken while observing the bladder wall either by means of a video camera, or by direct endoscopic observation. RESULTS: A good correlation was found between the PpIX fluorescence and the histopathological diagnosis. On a total of 215 biopsies, 143 in fluorescent and 72 in nonfluorescent areas, all visible tumors on white light cytoscopy appeared in a bright red fluorescence with the photodetection technique. In addition, this method permitted to discover 47 unsuspected carcinomatous lesions on white light observation, among which 40% were carcinoma in situ. CONCLUSION: PpIX fluorescence induced by instillation into the bladder of 5-ALA is an efficient method of mapping the mucosa in bladder carcinoma.
Keywords
Administration, Cutaneous, Administration, Intravesical, Adult, Aged, Aged, 80 and over, Aminolevulinic Acid, Carcinoma, Transitional Cell, Evaluation Studies as Topic, Female, Fluorescence, Humans, Male, Middle Aged, Photofluorography, Photosensitizing Agents, Protoporphyrins, Urinary Bladder Neoplasms
Pubmed
Web of science
Create date
21/01/2008 16:09
Last modification date
20/08/2019 13:40
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