N-Nitrosation Based Fluorescence Turn-On Nitric Oxide Probe: Kinetic and Cell Imaging Studies.
Details
Serval ID
serval:BIB_40F2CB4CCC11
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
N-Nitrosation Based Fluorescence Turn-On Nitric Oxide Probe: Kinetic and Cell Imaging Studies.
Journal
ACS applied bio materials
ISSN
2576-6422 (Electronic)
ISSN-L
2576-6422
Publication state
Published
Issued date
21/08/2023
Peer-reviewed
Oui
Volume
6
Number
8
Pages
3266-3277
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Nitric oxide (NO) is a ubiquitous messenger molecule playing a key role in various physiological and pathological processes. However, producing a selective turn-on fluorescence response to NO is a challenging task due to (a) the very short half-life of NO (typically in the range of 0.1-10 s) in the biological milieu and (b) false positive responses to reactive carbonyl species (RCS) (e.g., dehydroascorbic acid and methylglyoxal etc.) and some other reactive oxygen/nitrogen species (ROS/RNS), especially with o-phenylenediamine (OPD) based fluorosensors. To avoid these limitations, NO sensors should be designed in such a way that they react spontaneously with NO to give turn-on response within the time frame of t <sub>1/2</sub> (typically in the range of 0.1-10 s) of NO and λ <sub>em</sub> in the visible wavelength along with good cell permeability to achieve biocompatibility. With these views in mind, a N-nitrosation based fluorescent sensor, NDAQ, has been developed that is highly selective to NO with ∼27-fold fluorescence enhancement at λ <sub>em</sub> = 542 nm with high sensitivity (LOD = 7 ± 0.4 nM) and shorter response time, eliminating the interference of other reactive species (RCS/ROS/RNS). Furthermore, all the photophysical studies with NDAQ have been performed in 98% aqueous medium at physiological pH, indicating its good stability under physiological conditions. The kinetic assay illustrates the second-order dependency with respect to NO concentration and first-order dependency with respect to NDAQ concentration. The biological studies reveal the successful application of the probe to track both endogenous and exogenous NO in living organisms.
Keywords
Nitric Oxide, Reactive Oxygen Species, Nitrosation, Fluorescence, Reactive Nitrogen Species, Oxygen, DFT calculation, Endogenous detection, Exogenous detection, Kinetic studies, N-nitrosation mechanism, NO sensor
Pubmed
Web of science
Create date
10/08/2023 14:24
Last modification date
02/12/2023 8:15