TGF-beta receptor type II and fetuin in the developing sheep neocortex

Details

Serval ID
serval:BIB_40EF41B5507F
Type
Article: article from journal or magazin.
Collection
Publications
Title
TGF-beta receptor type II and fetuin in the developing sheep neocortex
Journal
Cell and Tissue Research
Author(s)
Dziegielewska  K. M., Williams  R. T., Knott  G. W., Kitchener  P. D., Monk  S. E., Potter  A., Saunders  N. R.
ISSN
0302-766X (Print)
Publication state
Published
Issued date
12/1997
Volume
290
Number
3
Pages
515-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
Fetuin shows a characteristic pattern of distribution in the developing neocortex in many mammalian species. Its expression is confined to early-appearing cortical-plate and later subplate neurons. A short 19 amino-acid sequence of fetuin shows a degree of homology to an 18 amino-acid sequence of the TGF-beta type II receptor (TbetaR-II) and in vitro fetuin binds to members of the TGF-beta family of cytokines. It has been suggested that fetuin is the biologically significant antagonist of these cytokines. We have compared, using immunocytochemistry, the distribution pattern of TbetaR-II and fetuin in the developing neocortex of foetal sheep. TbetaR-II immunoreactivity first appears at around 40 days of gestation in the fetal sheep (E40, term in sheep is 150 days from conception), localised in two discreet bands: one just outside the cortical plate in the inner part of the marginal zone and one deep in the cortical plate in what becomes the transient subplate zone. By E70-E80, TbetaR-II is prominent in a population of subplate cells, whereas, by E120 only small patches of TbetaR-II-positive cells are visible, principally in pyramidal cells in layer VI. The developmental sequence of the staining pattern for TbetaR-II in the neocortex is complementary to that for fetuin, rather than overlapping with it. Double-labelling of fetuin and TbetaR-II shows some cellular co-localisation, especially at E60, but most fetuin-positive cells are not immunoreactive for TbetaR-II. Thus, fetuin's proposed role as an antagonist of TGF-beta cytokines and mimic of TbetaR-II is not consistent with the observed distribution of these two molecules in the developing neocortex of the foetal sheep.
Keywords
Animals Fetus/metabolism Gestational Age Immunohistochemistry Neocortex/*embryology/*metabolism Receptors, Transforming Growth Factor beta/*metabolism Sheep Tissue Distribution Transforming Growth Factor beta/antagonists & inhibitors/metabolism alpha-Fetoproteins/*metabolism
Pubmed
Web of science
Create date
24/01/2008 14:26
Last modification date
20/08/2019 13:40
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