S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi.

Details

Serval ID
serval:BIB_40C4AA5B9D10
Type
Article: article from journal or magazin.
Collection
Publications
Title
S-phase synchronized CHO cells show elevated transfection efficiency and expression using CaPi.
Journal
Cytotechnology
Author(s)
Grosjean F., Batard P., Jordan M., Wurm F.M.
ISSN
0920-9069 (Print)
ISSN-L
0920-9069
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
38
Number
1-3
Pages
57-62
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Various methods exist to transfect mammalian cells in culture. It is generally accepted that individual methods have to be optimized for each of the cell lines or cell types used. Despitethe use of optimized protocols, significant day-to-day variationsin transfection efficiency regularly occur. We postulate that the;status' of cell populations prior to transfection is involved insuch variability. This study evaluates standardized transfectionsdone at different phases of the cell cycle. Cell synchronizationwas achieved using mimosine. Transfection efficiency was monitored by fluorescence quantification of GFP (Green Fluorescent Protein). We show that transfection using the calcium-phosphate-DNA co-precipitation method, at differentphases of the cell cycle, yields variable expression levels of GFP. Highest GFP expression levels were seen when transfecting cell populations with a dominant representation of S-phase-cells.
Pubmed
Web of science
Create date
06/03/2015 12:01
Last modification date
20/08/2019 13:39
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