A critical perspective of the diverse roles of O-GlcNAc transferase in chromatin.

Details

Serval ID
serval:BIB_408FD4758153
Type
Article: article from journal or magazin.
Collection
Publications
Title
A critical perspective of the diverse roles of O-GlcNAc transferase in chromatin.
Journal
Chromosoma
Author(s)
Gambetta M.C., Müller J.
ISSN
1432-0886 (Electronic)
ISSN-L
0009-5915
Publication state
Published
Issued date
12/2015
Peer-reviewed
Oui
Volume
124
Number
4
Pages
429-442
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Abstract
O-linked β-N-Acetylglucosamine (O-GlcNAc) is a posttranslational modification that is catalyzed by O-GlcNAc transferase (Ogt) and found on a plethora of nuclear and cytosolic proteins in animals and plants. Studies in different model organisms revealed that while O-GlcNAc is required for selected processes in Caenorhabditis elegans and Drosophila, it has evolved to become required for cell viability in mice, and this has challenged investigations to identify cellular functions that critically require this modification in mammals. Nevertheless, a principal cellular process that engages O-GlcNAcylation in all of these species is the regulation of gene transcription. Here, we revisit several of the primary experimental observations that led to current models of how O-GlcNAcylation affects gene expression. In particular, we discuss the role of the stable association of Ogt with the transcription factors Hcf1 and Tet, the two main Ogt-interacting proteins in nuclei of mammalian cells. We also critically evaluate the evidence that specific residues on core histones, including serine 112 of histone 2B (H2B-S112), are O-GlcNAcylated in vivo and discuss possible physiological effects of these modifications. Finally, we review our understanding of the role of O-GlcNAcylation in Drosophila, where recent studies suggest that the developmental defects in Ogt mutants are all caused by lack of O-GlcNAcylation of a single transcriptional regulator, the Polycomb repressor protein Polyhomeotic (Ph). Collectively, this reexamination of the experimental evidence suggests that a number of recently propagated models about the role of O-GlcNAcylation in transcriptional control should be treated cautiously.
Keywords
Animals, Chromatin/metabolism, DNA-Binding Proteins, Drosophila Proteins, Gene Expression Regulation, Histones/metabolism, Humans, N-Acetylglucosaminyltransferases/metabolism, N-Acetylglucosaminyltransferases/physiology, Polycomb Repressive Complex 1, Protein Processing, Post-Translational
Pubmed
Web of science
Open Access
Yes
Create date
19/07/2018 11:03
Last modification date
21/08/2019 6:35
Usage data