Dissemination of antibiotic resistance genes associated with the sporobiota in sediments impacted by wastewater.

Details

Serval ID
serval:BIB_408A948312E3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dissemination of antibiotic resistance genes associated with the sporobiota in sediments impacted by wastewater.
Journal
PeerJ
Author(s)
Paul C., Bayrychenko Z., Junier T., Filippidou S., Beck K., Bueche M., Greub G., Bürgmann H., Junier P.
ISSN
2167-8359 (Print)
ISSN-L
2167-8359
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
6
Pages
e4989
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Aquatic ecosystems serve as a dissemination pathway and a reservoir of both antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG). In this study, we investigate the role of the bacterial sporobiota to act as a vector for ARG dispersal in aquatic ecosystems. The sporobiota was operationally defined as the resilient fraction of the bacterial community withstanding a harsh extraction treatment eliminating the easily lysed fraction of the total bacterial community. The sporobiota has been identified as a critical component of the human microbiome, and therefore potentially a key element in the dissemination of ARG in human-impacted environments. A region of Lake Geneva in which the accumulation of ARG in the sediments has been previously linked to the deposition of treated wastewater was selected to investigate the dissemination of <i>tet</i> (W) and <i>sul</i> 1, two genes conferring resistance to tetracycline and sulfonamide, respectively. Analysis of the abundance of these ARG within the sporobiome (collection of genes of the sporobiota) and correlation with community composition and environmental parameters demonstrated that ARG can spread across the environment with the sporobiota being the dispersal vector. A highly abundant OTU affiliated with the genus <i>Clostridium</i> was identified as a potential specific vector for the dissemination of <i>tet</i> (W), due to a strong correlation with <i>tet</i> (W) frequency (ARG copy numbers/ng DNA). The high dispersal rate, long-term survival, and potential reactivation of the sporobiota constitute a serious concern in terms of dissemination and persistence of ARG in the environment.
Keywords
ARG, Dispersal, Seed bank, WWTP, sul1, tet(W)
Pubmed
Web of science
Open Access
Yes
Create date
09/07/2018 16:46
Last modification date
20/08/2019 13:39
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