CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_40674097E50D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CcrZ is a pneumococcal spatiotemporal cell cycle regulator that interacts with FtsZ and controls DNA replication by modulating the activity of DnaA.
Journal
Nature microbiology
Author(s)
Gallay C., Sanselicio S., Anderson M.E., Soh Y.M., Liu X., Stamsås G.A., Pelliciari S., van Raaphorst R., Dénéréaz J., Kjos M., Murray H., Gruber S., Grossman A.D., Veening J.W.
ISSN
2058-5276 (Electronic)
ISSN-L
2058-5276
Publication state
Published
Issued date
09/2021
Peer-reviewed
Oui
Volume
6
Number
9
Pages
1175-1187
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Most bacteria replicate and segregate their DNA concomitantly while growing, before cell division takes place. How bacteria synchronize these different cell cycle events to ensure faithful chromosome inheritance by daughter cells is poorly understood. Here, we identify Cell Cycle Regulator protein interacting with FtsZ (CcrZ) as a conserved and essential protein in pneumococci and related Firmicutes such as Bacillus subtilis and Staphylococcus aureus. CcrZ couples cell division with DNA replication by controlling the activity of the master initiator of DNA replication, DnaA. The absence of CcrZ causes mis-timed and reduced initiation of DNA replication, which subsequently results in aberrant cell division. We show that CcrZ from Streptococcus pneumoniae interacts directly with the cytoskeleton protein FtsZ, which places CcrZ in the middle of the newborn cell where the DnaA-bound origin is positioned. This work uncovers a mechanism for control of the bacterial cell cycle in which CcrZ controls DnaA activity to ensure that the chromosome is replicated at the right time during the cell cycle.
Keywords
Bacillus subtilis/cytology, Bacillus subtilis/genetics, Bacillus subtilis/metabolism, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cell Cycle, Cytoskeletal Proteins/genetics, Cytoskeletal Proteins/metabolism, DNA Replication, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Protein Binding, Streptococcus pneumoniae/cytology, Streptococcus pneumoniae/genetics, Streptococcus pneumoniae/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
20/08/2021 16:19
Last modification date
22/09/2021 6:38
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