Combined effects of GDNF, BDNF, and CNTF on motoneuron differentiation in vitro

Details

Serval ID
serval:BIB_4046D503FB72
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Combined effects of GDNF, BDNF, and CNTF on motoneuron differentiation in vitro
Journal
Journal of Neuroscience Research
Author(s)
Zurn  A. D., Winkel  L., Menoud  A., Djabali  K., Aebischer  P.
ISSN
0360-4012 (Print)
Publication state
Published
Issued date
04/1996
Volume
44
Number
2
Pages
133-41
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 15
Abstract
We have previously shown that glial cell line-derived neurotrophic factor (GDNF), in addition to promoting the survival of dopaminergic neurons in cultures from embryonic rat ventral mesencephalon,also increases the activity of choline acetyltransferase (ChAT) in the cranial motoneurons present in these cultures (Zurn et al.: Neuroreport 6:113-118, 1994). By using the intermediate filament protein peripherin as a motoneuron marker, we report here that GDNF increases the number of motoneurons as well as the length of their neurites. Brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) also promote ChAT activity, motoneuron survival, and neurite outgrowth in these cultures, but to varying degrees. Although these three molecules have similar effects on cultured motoneurons, we provide evidence for a distinct mode of action of GDNF, BDNF, and CNTF, since combinations of GDNF and BDNF, GDNF and CNTF, and BDNF and CNTF have either additive or synergistic effects on ChAT activity and motoneuron number. In addition to the previously described motoneuron-specific neurotrophic factors BDNF and CNTF, GDNF combined with the latter two factors may provide an important tool for the treatment of human motoneuron diseases such as amyotrophic lateral sclerosis and spinal muscular atrophy, both by increasing efficiency of treatment, and by decreasing the likelihood of deleterious side-effects.
Keywords
Animals Biological Markers Brain-Derived Neurotrophic Factor Cell Differentiation/drug effects Cell Survival/drug effects Cells, Cultured Choline O-Acetyltransferase/metabolism Ciliary Neurotrophic Factor Drug Interactions Embryo Eye Proteins/analysis/biosynthesis Glial Cell Line-Derived Neurotrophic Factor Humans Intermediate Filament Proteins/analysis/biosynthesis *Membrane Glycoproteins Mesencephalon/cytology Motor Neuron Disease Motor Neurons/drug effects/*physiology Nerve Growth Factors/*pharmacology Nerve Tissue Proteins/*pharmacology Neurites/drug effects/physiology/ultrastructure Neuropeptides/analysis/biosynthesis Phosphopyruvate Hydratase/analysis/metabolism Rats Recombinant Proteins/pharmacology
Pubmed
Web of science
Create date
28/01/2008 9:44
Last modification date
20/08/2019 14:38
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