Towards Quantification of Inflammation in Atherosclerotic Plaque in the Clinic - Characterization and Optimization of Fluorine-19 MRI in Mice at 3 T.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_40394E9CBB35
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Towards Quantification of Inflammation in Atherosclerotic Plaque in the Clinic - Characterization and Optimization of Fluorine-19 MRI in Mice at 3 T.
Journal
Scientific reports
Author(s)
Darçot E., Colotti R., Pellegrin M., Wilson A., Siegert S., Bouzourene K., Yerly J., Mazzolai L., Stuber M., van Heeswijk R.B.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
25/11/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
17488
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Fluorine-19 ( <sup>19</sup> F) magnetic resonance imaging (MRI) of injected perfluorocarbons (PFCs) can be used for the quantification and monitoring of inflammation in diseases such as atherosclerosis. To advance the translation of this technique to the clinical setting, we aimed to 1) demonstrate the feasibility of quantitative <sup>19</sup> F MRI in small inflammation foci on a clinical scanner, and 2) to characterize the PFC-incorporating leukocyte populations and plaques. To this end, thirteen atherosclerotic apolipoprotein-E-knockout mice received 2 × 200 µL PFC, and were scanned on a 3 T clinical MR system. <sup>19</sup> F MR signal was detected in the aortic arch and its branches in all mice, with a signal-to-noise ratio of 11.1 (interquartile range IQR = 9.5-13.1) and a PFC concentration of 1.15 mM (IQR = 0.79-1.28). Imaging flow cytometry was used on another ten animals and indicated that PFC-labeled leukocytes in the aortic arch and it branches were mainly dendritic cells, macrophages and neutrophils (ratio 9:1:1). Finally, immunohistochemistry analysis confirmed the presence of those cells in the plaques. We thus successfully used <sup>19</sup> F MRI for the noninvasive quantification of PFC in atherosclerotic plaque in mice on a clinical scanner, demonstrating the feasibility of detecting very small inflammation foci at 3 T, and advancing the translation of <sup>19</sup> F MRI to the human setting.
Pubmed
Web of science
Open Access
Yes
Create date
27/11/2019 14:28
Last modification date
29/07/2020 7:08
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