Nonself vegetative fusion and genetic exchange in the arbuscular mycorrhizal fungus Glomus intraradices.

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Serval ID
serval:BIB_401529471CE0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Nonself vegetative fusion and genetic exchange in the arbuscular mycorrhizal fungus Glomus intraradices.
Journal
New Phytologist
Author(s)
Croll D., Giovannetti M., Koch A.M., Sbrana C., Ehinger M., Lammers P.J., Sanders I.R.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
181
Number
4
Pages
924-937
Language
english
Abstract
Arbuscular mycorrhizal fungi (AMF) form symbioses with the majority of plants and form extensive underground hyphal networks simultaneously connecting the roots of different plant species. No empirical evidence exists for either anastomosis between genetically different AMF or genetic exchange.Five isolates of one population of Glomus intraradices were used to study anastomosis between hyphae of germinating spores. We show that genetically distinct AMF, from the same field, anastomose, resulting in viable cytoplasmic connections through which genetic exchange could potentially occur.Pairs of genetically different isolates were then co-cultured in an in vitro system.Freshly produced spores were individually germinated to establish new cultures.Using several molecular tools, we show that genetic exchange occurred between genetically different AMF. Specific genetic markers from each parent were transmitted to the progeny. The progeny were viable, forming symbioses with plant roots. The phenotypes of some of the progeny were significantly different from either parent.Our results indicate that considerable promiscuity could occur in these fungi because nine out of 10 combinations of different isolates anastomosed. The ability to perform genetic crosses between AMF experimentally lays a foundation for understanding the genetics and evolutionary biology of these important plants symbionts.
Keywords
anastomoses, arbuscular mycorrhizal fungi (AMF), crossing experiment, genetic exchange, genetic markers, Glomus intraradices, nonself vegetative fusion, phenotypic traits
Pubmed
Web of science
Open Access
Yes
Create date
11/11/2008 18:18
Last modification date
20/08/2019 13:37
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