Genome-wide RNA-seq analysis indicates that the DAG1 transcription factor promotes hypocotyl elongation acting on ABA, ethylene and auxin signaling.

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State: Public
Version: Final published version
Serval ID
serval:BIB_400691B66025
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genome-wide RNA-seq analysis indicates that the DAG1 transcription factor promotes hypocotyl elongation acting on ABA, ethylene and auxin signaling.
Journal
Scientific reports
Author(s)
Lorrai R., Gandolfi F., Boccaccini A., Ruta V., Possenti M., Tramontano A., Costantino P., Lepore R., Vittorioso P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
26/10/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
15895
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Hypocotyl elongation is influenced by light and hormones, but the molecular mechanisms underlying this process are not yet fully elucidated. We had previously suggested that the Arabidopsis DOF transcription factor DAG1 may be a negative component of the mechanism of light-mediated inhibition of hypocotyl elongation, as light-grown dag1 knock-out mutant seedlings show significant shorter hypocotyls than the wild type. By using high-throughput RNA-seq, we compared the transcriptome profile of dag1 and wild type hypocotyls and seedlings. We identified more than 250 genes differentially expressed in dag1 hypocotyls, and their analysis suggests that DAG1 is involved in the promotion of hypocotyl elongation through the control of ABA, ethylene and auxin signaling. Consistently, ChIP-qPCR results show that DAG1 directly binds to the promoters of WRKY18 encoding a transcription factor involved in ABA signaling, of the ethylene- induced gene ETHYLENE RESPONSE FACTOR (ERF2), and of the SMALL AUXIN UP RNA 67 (SAUR67), an auxin-responding gene encoding a protein promoting hypocotyl cell expansion.
Pubmed
Web of science
Open Access
Yes
Create date
08/11/2018 17:30
Last modification date
20/08/2019 14:37
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