Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles.

Details

Serval ID
serval:BIB_3FF790512885
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genome mapping of a LYST mutation in corn snakes indicates that vertebrate chromatophore vesicles are lysosome-related organelles.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Ullate-Agote A., Burgelin I., Debry A., Langrez C., Montange F., Peraldi R., Daraspe J., Kaessmann H., Milinkovitch M.C., Tzika A.C.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
20/10/2020
Peer-reviewed
Oui
Volume
117
Number
42
Pages
26307-26317
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Reptiles exhibit a spectacular diversity of skin colors and patterns brought about by the interactions among three chromatophore types: black melanophores with melanin-packed melanosomes, red and yellow xanthophores with pteridine- and/or carotenoid-containing vesicles, and iridophores filled with light-reflecting platelets generating structural colors. Whereas the melanosome, the only color-producing endosome in mammals and birds, has been documented as a lysosome-related organelle, the maturation paths of xanthosomes and iridosomes are unknown. Here, we first use 10x Genomics linked-reads and optical mapping to assemble and annotate a nearly chromosome-quality genome of the corn snake Pantherophis guttatus The assembly is 1.71 Gb long, with an N50 of 16.8 Mb and L50 of 24. Second, we perform mapping-by-sequencing analyses and identify a 3.9-Mb genomic interval where the lavender variant resides. The lavender color morph in corn snakes is characterized by gray, rather than red, blotches on a pink, instead of orange, background. Third, our sequencing analyses reveal a single nucleotide polymorphism introducing a premature stop codon in the lysosomal trafficking regulator gene (LYST) that shortens the corresponding protein by 603 amino acids and removes evolutionary-conserved domains. Fourth, we use light and transmission electron microscopy comparative analyses of wild type versus lavender corn snakes and show that the color-producing endosomes of all chromatophores are substantially affected in the LYST mutant. Our work provides evidence characterizing xanthosomes in xanthophores and iridosomes in iridophores as lysosome-related organelles.
Keywords
LYST, chromatophores, corn snake, lysosome-related organelles, pigmentation
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2020 13:58
Last modification date
07/07/2021 6:36
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