Activation of the MHC class II transactivator CIITA by interferon-gamma requires cooperative interaction between Stat1 and USF-1.

Details

Serval ID
serval:BIB_3FDC97BD951B
Type
Article: article from journal or magazin.
Collection
Publications
Title
Activation of the MHC class II transactivator CIITA by interferon-gamma requires cooperative interaction between Stat1 and USF-1.
Journal
Immunity
Author(s)
Muhlethaler-Mottet A, Di Berardino W, Otten LA, Mach B
Publication state
Published
Issued date
02/1998
Peer-reviewed
Oui
Language
english
Abstract
CIITA is the mediator of MHC class II gene induction by interferon-γ (IFNγ). The CIITA gene is itself selectively activated via one of its four promoters (PIV). We show here that three cis-acting elements, the GAS, the E box, and the IRF-1–binding site, as well as the trans-acting factors Stat1 and IRF-1, are essential for activation of CIITA promoter IV by IFNγ. Stat1 binds to the GAS site only in the presence of the ubiquitous factor USF-1, which binds to the adjacent E box. Indeed, Stat1 and USF-1 bind to the GAS/E box motif in a cooperative manner. The specificity for CIITA activation by IFNγ is thus dictated by the GAS/E box motif and by the selective interaction of IFNγ-activated Stat1 and USF-1. This clarifies the missing link in the overall pathway of IFNγ activation of MHC-II expression.
Pubmed
Open Access
Yes
Create date
23/03/2020 12:44
Last modification date
25/03/2020 7:26
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