A study of the alkaline proteases secreted by different Aspergillus species

Details

Serval ID
serval:BIB_3FA7B23EE81C
Type
Article: article from journal or magazin.
Collection
Publications
Title
A study of the alkaline proteases secreted by different Aspergillus species
Journal
Mycoses
Author(s)
Hanzi  M., Shimizu  M., Hearn  V. M., Monod  M.
ISSN
0933-7407 (Print)
Publication state
Published
Issued date
12/1993
Volume
36
Number
11-12
Pages
351-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov-Dec
Abstract
Strains from several species of Aspergillus were grown in the presence of soluble collagen, and the major secreted proteins present in the culture fluid were examined for proteolytic activity. The possibility of relatedness among the alkaline proteases secreted by Aspergillus was studied by probing extracts from the various species with polyclonal antisera raised to the isolated alkaline proteases of A. fumigatus and A. oryzae. The pathogenic species A. flavus, A. terreus and A. nidulans hydrolyse collagen and were found to secrete an alkaline protease related to that of Aspergillus fumigatus. In contrast, A. niger and non-pathogenic species such A. glaucus, A. versicolor and A. clavatus were unable to degrade collagen in vitro. These findings suggest a possible pathogenic role for the secreted alkaline proteases of Aspergillus species.
Keywords
Aspergillus/*enzymology Collagen/pharmacology Endopeptidases/*metabolism Immunoblotting Species Specificity
Pubmed
Web of science
Create date
25/01/2008 17:47
Last modification date
03/03/2018 16:25
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