Differential expression and modification of neurofilament triplet proteins during cat cerebellar development.

Details

Serval ID
serval:BIB_3F9C981DE6CF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential expression and modification of neurofilament triplet proteins during cat cerebellar development.
Journal
Journal of Comparative Neurology
Author(s)
Riederer B.M., Porchet R., Marugg R.A.
ISSN
0021-9967 (Print)
ISSN-L
0021-9967
Publication state
Published
Issued date
1996
Volume
364
Number
4
Pages
704-717
Language
english
Abstract
Neurofilament (NF) proteins consist of three subunits of different molecular weights defined as NF-H, NF-M, and NF-L. They are typical structures of the neuronal cytoskeleton. Their immunocytochemical distribution during postnatal development of cat cerebellum was studied with several monoclonal and polyclonal antibodies against phosphorylated or unmodified sites. Expression and distribution of the triplet neurofilament proteins changed with maturation. Afferent mossy and climbing fibers in the medullary layer contained NF-M and NF-L already at birth, whereas NF-H appeared later. Within the first three postnatal weeks, all three subunits appeared in mossy and climbing fibers in the internal granular and molecular layers and in the axons of Purkinje cells. Axons of local circuit neurons such as basket cells expressed these proteins at the end of the first month, whereas parallel fibers expressed them last, at the beginning of the third postnatal month. Differential localization was especially observed for NF-H. Depending on phosphorylation, NF-H proteins were found in different axon types in climbing, mossy, and basket fibers or additionally in parallel fibers. A nonphosphorylated NF-H subunit was exclusively located in some Purkinje cells at early developmental stages and in some smaller interneurons later. A novel finding is the presence of a phosphorylation site in the NF-H subunit that is localized in dendrites of Purkinje cells but not in axons. Expression and phosphorylation of the NF-H subunit, especially, is cell-type specific and possibly involved in the adult-type stabilization of the axonal and dendritic cytoskeleton.
Keywords
Animals, Antibodies, Monoclonal/diagnostic use, Cats, Cerebellum/cytology, Cerebellum/growth & development, Electrophoresis, Polyacrylamide Gel, Female, Immunoblotting, Immunohistochemistry, Male, Neurofilament Proteins/biosynthesis, Neurofilament Proteins/metabolism, Phosphorylation, Protein Processing, Post-Translational/physiology, Purkinje Cells/metabolism, Purkinje Cells/physiology, Spinal Cord/growth & development, Spinal Cord/metabolism
Pubmed
Web of science
Create date
24/01/2008 15:35
Last modification date
20/08/2019 14:36
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