The effect of standard and high-fluence corneal cross-linking (CXL) on cornea and limbus.

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State: Public
Version: author
Serval ID
serval:BIB_3F78479A7B8E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The effect of standard and high-fluence corneal cross-linking (CXL) on cornea and limbus.
Journal
Investigative Ophthalmology and Visual Science
Author(s)
Richoz O., Tabibian D., Hammer A., Majo F., Nicolas M., Hafezi F.
ISSN
1552-5783 (Electronic)
ISSN-L
0146-0404
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
55
Number
9
Pages
5783-5787
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.Publication Status: epublish
Abstract
PURPOSE: When treating peripheral ectatic disease-like pellucid marginal degeneration (PMD), corneal cross-linking with UV-A and riboflavin (CXL) must be applied eccentrically to the periphery of the lower cornea, partly irradiating the corneal limbus. Here, we investigated the effect of standard and double-standard fluence corneal cross-linking with riboflavin and UV-A (CXL) on cornea and corneal limbus in the rabbit eye in vivo.
METHODS: Epithelium-off CXL was performed in male New Zealand White rabbits with two irradiation diameters (7 mm central cornea, 13 mm cornea and limbus), using standard fluence (5.4 J/cm(2)) and double-standard fluence (10.8 J/cm(2)) settings. Controls were subjected to epithelial removal and riboflavin instillation, but were not irradiated with UV-A. Following CXL, animals were examined daily until complete closure of the epithelium, and at 7, 14, 21, and 28 days. Animals were killed and a corneoscleral button was excised and processed for light microscopy and immunohistochemistry.
RESULTS: For both irradiation diameters and fluences tested, no signs of endothelial damage or limbal vessel thrombosis were observed, and time to re-epithelialization was similar to untreated controls. Histological and immunohistochemical analysis revealed no differences in the p63 putative stem cell marker expression pattern.
CONCLUSIONS: Even when using fluence twice as high as the one used in current clinical CXL settings, circumferential UV-A irradiation of the corneal limbus does not alter the regenerative capacity of the limbal epithelial cells, and the expression pattern of the putative stem cell marker p63 remains unchanged. This suggests that eccentric CXL may be performed safely in PMD.
Keywords
Animals, Biological Markers/metabolism, Cornea/drug effects, Cornea/metabolism, Cross-Linking Reagents/pharmacology, Epithelium, Corneal/metabolism, Immunohistochemistry, Keratin-3/metabolism, Limbus Corneae/drug effects, Limbus Corneae/metabolism, Male, Photosensitizing Agents/pharmacology, Rabbits, Riboflavin/pharmacology, Transcription Factors/metabolism, Ultraviolet Rays
Pubmed
Web of science
Open Access
Yes
Create date
11/08/2014 12:03
Last modification date
20/08/2019 14:36
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