Myocardial angiogenesis induction with bone protein derived growth factors (animal experiment).

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Version: Final published version
Serval ID
serval:BIB_3F421906B1E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Myocardial angiogenesis induction with bone protein derived growth factors (animal experiment).
Journal
Swiss Medical Weekly
Author(s)
Mueller X.M., Tevaearai H.T., Genton C.Y., Chaubert P., von Segesser L.K.
ISSN
1424-7860 (Print)
ISSN-L
0036-7672
Publication state
Published
Issued date
2001
Volume
131
Number
1-2
Pages
23-25
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Myocardial angiogenesis induction with vascular growth factors constitutes a potential strategy for patients whose coronary artery disease is refractory to conventional treatment. The importance of angiogenesis in bone formation has led to the development of growth factors derived from bovine bone protein. Twelve pigs (mean weight, 73 +/- 3 kg) were chosen for the study. In the first group (n = 6, growth factor group) five 100 micrograms boluses of growth factors derived from bovine bone protein, diluted in Povidone 5%, were injected in the lateral wall of the left ventricle. In the second group (n = 6, control group), the same operation was performed but only the diluting agent was injected. All the animals were sacrificed after 28 days and the vascular density of the left lateral wall (expressed as the number of vascular structures per mm2) as well as the area of blood vessel profiles per myocardial area analysed were determined histologically with a computerised system. The growth factor group had a capillary density which was significantly higher than that of the control group: 12.6 +/- 0.9/mm2 vs 4.8 +/- 0.5/mm2 (p < 0.01). The same holds true for the arteriolar density: 1 +/- 0.2/mm2 vs 0.3 +/- 0.1/mm2 (p < 0.01). The surface ratios of blood vessel profiles per myocardial area were 4900 +/- 800 micron 2/mm2 and 1550 +/- 400 micron 2/mm2 (p < 0.01) respectively. In this experimental model, bovine bone protein derived growth factors induce a significant neovascularisation in healthy myocardium, and appear therefore as promising candidates for therapeutic angiogenesis.
Keywords
Animals, Bone and Bones/chemistry, Coronary Circulation/drug effects, Coronary Circulation/physiology, Coronary Vessels/drug effects, Coronary Vessels/physiology, Factor VIII/analysis, Growth Substances/isolation & purification, Growth Substances/pharmacology, Neovascularization, Physiologic/drug effects, Neovascularization, Physiologic/physiology, Povidone, Proteins/isolation & purification, Proteins/pharmacology, Swine
Pubmed
Web of science
Create date
14/02/2008 15:19
Last modification date
20/08/2019 14:36
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