Identification of the master sex determining gene in Northern pike (Esox lucius) reveals restricted sex chromosome differentiation.

Details

Serval ID
serval:BIB_3EE6D5FEDE8A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of the master sex determining gene in Northern pike (Esox lucius) reveals restricted sex chromosome differentiation.
Journal
PLoS genetics
Author(s)
Pan Q., Feron R., Yano A., Guyomard R., Jouanno E., Vigouroux E., Wen M., Busnel J.M., Bobe J., Concordet J.P., Parrinello H., Journot L., Klopp C., Lluch J., Roques C., Postlethwait J., Schartl M., Herpin A., Guiguen Y.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
08/2019
Peer-reviewed
Oui
Volume
15
Number
8
Pages
e1008013
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities to study the formation of sex chromosomes and the mechanisms driving the birth of new master sex determining (MSD) genes. However, the evolutionary interplay between the sex chromosomes and the MSD genes they harbor is rather unexplored. We characterized a male-specific duplicate of the anti-Müllerian hormone (amh) as the MSD gene in Northern Pike (Esox lucius), using genomic and expression evidence as well as by loss-of-function and gain-of-function experiments. Using RAD-Sequencing from a family panel, we identified Linkage Group (LG) 24 as the sex chromosome and positioned the sex locus in its sub-telomeric region. Furthermore, we demonstrated that this MSD originated from an ancient duplication of the autosomal amh gene, which was subsequently translocated to LG24. Using sex-specific pooled genome sequencing and a new male genome sequence assembled using Nanopore long reads, we also characterized the differentiation of the X and Y chromosomes, revealing a small male-specific insertion containing the MSD gene and a limited region with reduced recombination. Our study reveals an unexpectedly low level of differentiation between a pair of sex chromosomes harboring an old MSD gene in a wild teleost fish population, and highlights both the pivotal role of genes from the amh pathway in sex determination, as well as the importance of gene duplication as a mechanism driving the turnover of sex chromosomes in this clade.
Keywords
Animals, Animals, Genetically Modified, Anti-Mullerian Hormone/genetics, Chromosome Mapping, Esocidae/physiology, Female, Gene Duplication, Gene Knockdown Techniques, Male, Phylogeny, Sex Chromosomes/genetics, Sex Determination Processes/genetics, Synteny
Pubmed
Web of science
Open Access
Yes
Create date
05/09/2019 10:24
Last modification date
11/01/2020 7:16
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