CD103 marks a subset of human CD34(+)-derived langerin(+) dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1.

Details

Serval ID
serval:BIB_3E4DC6B75EB2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD103 marks a subset of human CD34(+)-derived langerin(+) dendritic cells that induce T-regulatory cells via indoleamine 2,3-dioxygenase-1.
Journal
Experimental Hematology
Author(s)
Očadlíková D., Trabanelli S., Salvestrini V., Ciciarello M., Evangelisti C., Lecciso M., Sabattini E., Righi S., Piccioli M., Pileri S.A., Lemoli R.M., Curti A.
ISSN
1873-2399 (Electronic)
ISSN-L
0301-472X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
43
Number
4
Pages
268-276.e5
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Indoleamine 2,3-dioxygenase 1 (IDO1) is an immunosuppressive molecule expressed in some subsets of normal and neoplastic cells. Mature human dendritic cells (DCs) have been shown to express IDO1, but little is known about its expression and function during DC differentiation from bone marrow hematopoietic stem/progenitor cells (HSPCs). Here, we show that during in vitro differentiation along the myeloid DC lineage, CD34(+) HSPCs acquire IDO1 expression, which acts in a tolerogenic manner by inducing a population of fully functional CD4(+)CD25(+) FOXP3(+) T-regulatory cells. Phenotypically, CD1a(+)CD14(-) HPSC-derived DCs expressed IDO1, langerin, CD11b, and CD1c. Cell-sorting experiments demonstrated that IDO1 expression is found in a subset of CD1a(+)CD14(-)langerin(+) cells, expressing CD103, which is capable of inducing T-regulatory cells in an IDO1-dependent manner. In conclusion, DC differentiation from CD34(+) HSPCs results in the expression of a functionally active IDO1 protein in CD1a(+)langerin(+), CD103-expressing DCs. These data point toward IDO1 expression as part of a tolerogenic signature during DC development.
Pubmed
Web of science
Create date
11/05/2015 12:37
Last modification date
20/08/2019 13:34
Usage data