Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarized <sup>13</sup>C magnetic resonance.

Details

Serval ID
serval:BIB_3D4D5F06B036
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarized <sup>13</sup>C magnetic resonance.
Journal
Scientific reports
Author(s)
Can E., Mishkovsky M., Yoshihara HAI, Kunz N., Couturier D.L., Petrausch U., Doucey M.A., Comment A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
13/01/2020
Peer-reviewed
Oui
Volume
10
Number
1
Pages
200
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The metabolic shift induced in human CD4 <sup>+</sup> T lymphocytes by stimulation is characterized by an upregulation of glycolysis, leading to an augmentation in lactate production. This adaptation has already been highlighted with various techniques and reported in several previous studies. We herein propose a method to rapidly and noninvasively detect the associated increase in flux from pyruvate to lactate catalyzed by lactate dehydrogenase using hyperpolarized <sup>13</sup> C magnetic resonance, a technique which can be used for in vivo imaging. It was shown that the conversion of hyperpolarized <sup>13</sup> C-pyruvate to <sup>13</sup> C-lactate during the one-minute measurement increased by a mean factor of 3.6 in T cells stimulated for 5 days as compared to resting T cells. This method can be extended to other metabolic substrates and is therefore a powerful tool to noninvasively analyze T cell metabolism, possibly in vivo.
Keywords
Adult, Electrocardiography, Female, Fetal Monitoring, Heart Block/blood, Heart Block/etiology, Heart Block/physiopathology, Heart Rate, Fetal, Humans, Hypokalemia, Infant, Newborn, Potassium/blood, Pregnancy, Pregnancy Complications, Tachycardia/blood, Tachycardia/diagnosis, Tachycardia/etiology
Pubmed
Open Access
Yes
Create date
17/01/2020 17:13
Last modification date
23/10/2020 6:23
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