Changes in gene expression profile in human subcutaneous adipose tissue during significant weight loss.

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State: Public
Version: author
Serval ID
serval:BIB_3D2AA9140B46
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Changes in gene expression profile in human subcutaneous adipose tissue during significant weight loss.
Journal
Obesity Facts
Author(s)
Leyvraz C., Verdumo C., Suter M., Paroz A., Calmes J.M., Marques-Vidal P.M., Giusti V.
ISSN
1662-4033 (Electronic)
ISSN-L
1662-4025
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
5
Number
3
Pages
440-451
Language
english
Notes
Publication types: Journal Article
Abstract
Objective: To analyze the expression of peroxisome proliferator-activated receptor-γ1 and 2 (PPARγ1 and 2), 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), and leptin in adipose tissue (AT) of obese women during weight loss following Roux-en-Y gastric bypass (RYGB) and to compare these levels with those obtained in AT of nonobese subjects. Methods: Gene expression was determined by real-time RT-PCR prior to surgery and at 3, 6, and 12 months after RYGB. Results: All obese patients lost weight, reaching a mean BMI of 29.3 ± 1.0 kg/m(2) at 1 year after surgery (-33.9 ± 1.5% of their initial body weight). In obese subjects leptin and 11βHSD1 were over-expressed, whereas PPARγ1 was expressed at lower levels compared to controls. After surgery, leptin and 11βHSD1 gene expression decreased, whereas PPARγ1 expression increased. At 12 months after RYGB, these 3 genes had reached levels similar to the controls. In contrast, PPARγ2 gene expression was not different between groups and types of tissue and remained unchanged during weight loss. We found a positive correlation between BMI and levels of gene expression of leptin and 11βHSD1. Conclusion: Gene expression of leptin, PPARγ1, and 11βHSD1 in AT is modified in human obesity. This default is completely corrected by RYGB. Copyright © 2012 S. Karger GmbH, Freiburg.
Pubmed
Web of science
Open Access
Yes
Create date
29/07/2012 15:24
Last modification date
20/08/2019 14:33
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