Decision Making in Phagocytosis.

Details

Serval ID
serval:BIB_3CCDAECBDE69
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Decision Making in Phagocytosis.
Journal
Advances in experimental medicine and biology
Author(s)
Prassler J., Simon F., Ecke M., Gruber S., Gerisch G.
ISSN
0065-2598 (Print)
ISSN-L
0065-2598
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
1246
Pages
71-81
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Dictyostelium cells are professional phagocytes that are capable of handling particles of variable shapes and sizes. Here we offer long bacteria that challenge the uptake mechanism to its limits and report on the responses of the phagocytes if they are unable to engulf the particle by closing the phagocytic cup. Reasons for failure may be a length of the particle much larger than the phagocyte's diameter, or competition with another phagocyte. A cell may simultaneously release a particle and engulf another one. The final phase of release can be fast, causing the phagosome membrane to turn inside-out and to form a bleb. Myosin-II may be involved in the release by generating tension at the plasma membrane, it does however not accumulate on the phagosome to act there directly in expelling the particle. Labeling with GFP-2FYVE indicates that processing of the phagosome with phosphatidylinositol 3-phosphate begins at the base of a long phagosome already before closure of the cup. The decision of releasing the particle can be made even at the stage of the processed phagosome.
Keywords
Bacteria/cytology, Dictyostelium/cytology, Phagocytes/cytology, Phagocytosis, Phagosomes/metabolism, Dictyostelium, actin, membrane blebbing, myosin-II, phosphatidylinositol (3,4,5)-trisphosphate, phosphatidylinositol 3-phosphate, tubular phagocytic cup
Pubmed
Create date
14/06/2020 20:59
Last modification date
27/10/2020 6:26
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